猪伪狂犬病毒和猪细小病毒双重PCR方法的建立及初步应用  

The Establishment and Application of a Duplex PCR Method for Detection of Pseudorabies Virus and Porcine Parvovirus

作　　者：夏应菊[1] 汪葆玥[1] 訾占超[1] 韩雪[1] 曲萍[1] 倪建强[1] 顾小雪[1] 遇秀玲[1] 翟新验[1] 田克恭[1] 

机构地区：[1]中国动物疫病预防控制中心,北京100125

出　　处：《中国动物检疫》2011年第12期32-35,共4页China Animal Health Inspection

基　　金："北京市生猪产业技术体系创新团队"课题(GWZJ-2009-05)

摘　　要：目的本研究旨在建立一种适用于临床样品和动物源性生物制品中猪伪狂犬病毒和猪细小病毒同时检测的双重PCR技术。方法针对猪伪狂犬病毒(PRV)的gE基因和猪细小病毒(PPV)的VP2基因的保守区域分别设计引物。结果经条件优化后,所建立的双重PCR方法能特异性地检测出样品中的PRV(581bp)和PPV(202bp)。结论本方法具有良好的特异性、敏感性和稳定性,适用于临床样品中对PRV和PPV的同时检测,也可用于猪源性生物制品的检测。Objootivo A duplex PCR method was developed to detect pseudorabies virus （PRV） and porcine parvovirus （PPV） . Method Two sets of specific primers were designed according to the conservative sequence of gE of PRV and VP2 of PPV individually. Based on the optimization of PCR, the duplex PCR would applied to detect the nucleic acid of PRV with 581 bp and PPV with 202 bp.Conclusion The results indicated that the duplex PCR was characterized by good sensitivity, specificity and stability, and could be used for not only clinical diagnosis but also biologic products detection.

关 键 词：双重PCR 猪伪狂犬病毒 猪细小病毒 生物制品 

分 类 号：S852.659.1[农业科学—基础兽医学]