淀粉污泥酸处理产氢特性及微生物多样性研究  

Effect of Acid Pretreatment on Fermentative Hydrogen Production and Microbial Diversity from Starch Sludge

作　　者：邓慧[1] 王广策[2] 才金玲[3] 邓超冰 程夕冉[5] 黄良美 冼萍[1] 郭辰 

机构地区：[1]广西大学环境学院,广西南宁530004 [2]中国科学院海洋研究所,山东青岛266071 [3]天津科技大学海洋科学与工程学院,天津300457 [4]广西壮族自治区环境监测中心站,广西南宁530004 [5]广西大学农学院,广西南宁530004

出　　处：《环境科学与技术》2011年第12期178-180,共3页Environmental Science & Technology

基　　金：广西自然科学基金项目(桂科自0991191)

摘　　要：以淀粉厂厌氧活性污泥为对象,探讨了经酸处理后污泥的产氢特性。结果表明,酸处理后的活性污泥反应系统,其产氢量均大于未经处理的活性污泥反应系统,其中最适产氢酸处理pH值为4.0、处理时间为0.5 h。通过细胞裂解直接提取活性污泥细菌基因组DNA,PCR扩增细菌16S rRNA基因V3区和变性梯度凝胶电泳分离,获得酸处理活性污泥微生物群落的特征DNA指纹图谱。Effect of acid pretreatment on activated sludge for hydrogen production from glucose was investigated in batch cultures. Untreated inocula were shown to produce less amount of hydrogen than the pretreated one, with the optimal conditions of pH 4.0 and pretreatment time for 0.5h. To reveal microbial diversity of acid pretreated sludge, sludge in different conditions were sampled and their genomic DNA of microbial community was extracted directly, then 16S rRNA gene （V3 region） were amplified by using universal primers. These amplified DNA fragments were separated by denaturing gradient gel electrophoresis （DGGE）.

关 键 词：淀粉污泥 酸处理 发酵产氢 微生物多样性 

分 类 号：X703[环境科学与工程—环境工程]