^(13)C-methacetin breath test reproducibility study reveals persistent CYP1A2 stimulation on repeat examinations  

作　　者：Anna Kasicka-Jonderko Anna Nita Krzysztof Jonderko Magdalena Kamińska Barbara Bońska-Fajfrowska 

机构地区：[1]Department of Basic Biomedical Science, School of Pharmacy, Medical University of Silesia

出　　处：《World Journal of Gastroenterology》2011年第45期4979-4986,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by The Medical University of Silesia, contracts No. NN-1-106-06, KNW-1-043/08 and KNW-1-154/09

摘　　要：AIM: To find the most reproducible quantitative parameter of a standard 13C-methacetin breath test (13C-MBT). METHODS: Twenty healthy volunteers (10 female, 10 male) underwent the 13C-MBT after intake of 75 mg 13C-methacetin p.o. on three occasions. Short-and medium-term reproducibility was assessed with paired examinations taken at an interval of 2 and 18 d (medians), respectively. RESULTS: The reproducibility of the 1-h cumulative 13C recovery (AUC0-60), characterized by a coefficient of variation of 10%, appeared to be considerably better than the reproducibility of the maximum momentary 13C recovery or the time of reaching it. Remarkably, as opposed to the short gap between consecutive examinations, the capacity of the liver to handle 13C-methacetin increased slightly but statistically significantly when a repeat dose was administered after two to three weeks.Regarding the AUC0-60, the magnitude of this fixed bias amounted to 7.5%. Neither the time gap between the repeat examinations nor the gender of the subjects affected the 13C-MBT reproducibility. CONCLUSION: 13C-MBT is most reproducibly quantified by the cumulative 13C recovery, but the exactitude thereof may be modestly affected by persistent stimulation of CYP1A2 on repeat examinations.AIM： To find the most reproducible quantitative parameter of a standard 13C-methacetin breath test （13C-MBT）. METHODS： Twenty healthy volunteers （10 female, 10 male） underwent the 13C-MBT after intake of 75 mg 13C-methacetin p.o. on three occasions. Short-and medium-term reproducibility was assessed with paired examinations taken at an interval of 2 and 18 d （medians）, respectively. RESULTS： The reproducibility of the 1-h cumulative 13C recovery （AUC0-60）, characterized by a coefficient of variation of 10%, appeared to be considerably better than the reproducibility of the maximum momentary 13C recovery or the time of reaching it. Remarkably, as opposed to the short gap between consecutive examinations, the capacity of the liver to handle 13C-methacetin increased slightly but statistically significantly when a repeat dose was administered after two to three weeks.Regarding the AUC0-60, the magnitude of this fixed bias amounted to 7.5%. Neither the time gap between the repeat examinations nor the gender of the subjects affected the 13C-MBT reproducibility. CONCLUSION： 13C-MBT is most reproducibly quantified by the cumulative 13C recovery, but the exactitude thereof may be modestly affected by persistent stimulation of CYP1A2 on repeat examinations.

关 键 词：13C-Methacetin Breath test Isotope application in medicine Liver REPRODUCIBILITY 

分 类 号：R575.2[医药卫生—消化系统]