烟梗废料固态发酵生产苏云金芽孢杆菌的适宜条件筛选  

Screening of Solid State Fermentation Conditions of Waste Stem for Producing Bacillus Thuringiensis

作　　者：杜雷[1] 赵高岭[1] 席宇[1] 臧晓静[1] 朱大恒[1] 

机构地区：[1]郑州大学生物工程系

出　　处：《烟草科技》2011年第12期69-72,共4页Tobacco Science & Technology

基　　金：河南省烟草公司资助项目"烟草基质复合生物杀虫剂的研究与开发"(HYKJ200912)

摘　　要：以烟草废料烟梗为培养基主要原料,采用正交和单因素试验方法,对苏云金芽孢杆菌HD-1菌株(Bacillus thuringiensis HD-1)的固态发酵培养基及培养条件进行了优化。结果表明,烟梗固态发酵培养基的最佳组合为:烟梗88.13%,酵母粉1.17%,蛋白胨1.17%,豆饼粉5.88%,玉米粉2.93%,KH2PO40.18%,MgSO4.7H2O 0.24%,FeSO4 0.12%和MnSO4 0.18%;最佳培养条件为:接种量6%,初始含水率65%,初始pH7.5。在这种发酵条件下的产孢量可稳定在9.5×1010 CFU/g。因此,利用烟梗废料固态发酵生产苏云金芽孢杆菌是可行的。Using waste tobacco stem as raw material, the medium compositions and conditions of solid state fermentation for Bacillus thuringiensis HD-1 growth were optimized by orthogonal design and single factor experiments. The results showed that the optimal medium composition was： tobacco stem 88.13%, dusty yeast 1.17%, peptone 1.17%, ground soybean cake 5.88%, corn flour 2.93%, KH2PO4 0.18%, MgSO4· 7H2O 0.24%, FeSO4 0.12% and MnSO4 0.18%. The best culture conditions were： inoculation concentration 6%, initial water content 65%,initial pH of the medium 7.5,under which sporulation stabilized at 9.5×10^10 CFU/g. It was feasible to produce Bacillus thuringiensis by solid state fermentation using waste tobacco stem as the main medium.

关 键 词：苏云金芽孢杆菌 固态发酵 正交设计 烟梗 产孢量 

分 类 号：TS49[农业科学—烟草工业]