HPLC-DAD法测定商品苦参根茎和根中5个生物碱的含量  

HPLC-DAD determination of 5 alkaloids in commercial available Kushen's rhizome and root

作　　者：张萍[1] 杨璐[2] 王耀欣[2] 殷其蕾[2] 贺锐锐[2] 詹先王[2] 刘勇[2] 袁丽佳[2] 李中杰[2] 韩南银[2] 

机构地区：[1]首都医科大学附属北京友谊医院中药剂科,北京100050 [2]北京大学药学院,北京100191

出　　处：《药物分析杂志》2011年第12期2294-2297,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定商品苦参根茎和根中苦参碱、氧化苦参碱、槐果碱、氧化槐果碱、槐定碱的含量,主要了解苦参根茎中苦参碱和氧化苦参碱总量是否达到中国药典2010年版规定的不低于1.0%。方法:采用Welch Materials XtimateTM C18(4.6 mm×150mm,5μm)色谱柱,流动相A为10 mmol.L-1醋酸铵水溶液(0.1%氨水调至pH 9.2),流动相B为乙腈,梯度洗脱,流速1 mL.min-1,检测波长220 nm。结果:苦参碱进样量在0.1575～2.205μg(r=0.9999),氧化苦参碱在0.8985～12.58μg(r=0.9999),槐果碱在0.1360～1.904μg(r=0.9999),氧化槐果碱在0.4398～6.153μg(r=0.9998),槐定碱在0.2092～2.926μg(r=0.9998)范围内呈良好的线性关系;加样平均回收率(n=3)分别为101.0%～102.5%(RSD≤2.1%),100.8%～101.6%(RSD≤2.8%),102.3%～103.6%(RSD≤1.2%),102.2%～103.4%(RSD≤1.1%),102.4%～103.3%(RSD≤2.9%)。结论:该方法精密度和准确度均好。苦参根茎和根中苦参碱和氧化苦参碱的总量均不低于1.0%,符合中国药典2010年版的规定。Objective：To determine the respective content of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine in commercial Kushen＇s rhizomes and roots so as to find out if the total contents of matrine and oxymatrin meet the request of not less than 1% in ChP（Vol.I）2010 Ed.Methods：The procedure was performed on a C18（4.6 mm×150 mm,5 μm） column with a gradient elution of mobile phase A（10 mmol·L-1 ammonium acetate aqueous,adjusted to pH 9.2 with ammonia water） and mobile phase B（acetonitrile）.The flow rate was 1 mL·min-1 and the detective wavelength of DAD was set at 220 nm.Results：The linear ranges of matrine,oxymatrin,sophocarpine,oxysophocarpine and sophoridine were 0.1575-2.205 μg（r=0.9999）,0.8985-12.58 μg（r=0.9999）,0.1360-1.904 μg（r=0.9999）,0.4398-6.153 μg（r=0.9998）,and 0.2092-2.926 μg（r=0.9998）,respectively;The recoveries（n=3） were 101.0%-102.5%（RSD≤2.1%）,100.8%-101.6%（RSD≤2.8%）,102.3%-103.6%（RSD≤1.2%）,102.2%-103.4%（RSD≤1.1%）,and 102.4%-103.3%（RSD≤2.9%）,respectively.Conclusion：The HPLC method shows a good precision and accuracy.The total contents of matrine and oxymatrine in both Kushen＇s rhizomes and roots are not less than 1.0%,which meet thestandardsin ChP（Vol.I）2010 Ed.

关 键 词：苦参 根茎 根 生物碱 苦参碱 氧化苦参碱 槐果碱 氧化槐果碱 槐定碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]