慢性应激抑郁大鼠模型的交感神经再生与心脏局部组织动作电位改变  

Change of heart tissue action potential and sympathetic nerve regeneration in chronic stress rat model

作　　者：刘政疆[1] 孙娟[1] 杨兰[2] 

机构地区：[1]新疆维吾尔自治区人民医院急救中心,乌鲁木齐832000 [2]新疆石河子大学病理教研室

出　　处：《中国心血管杂志》2011年第6期447-451,共5页Chinese Journal of Cardiovascular Medicine

摘　　要：目的探讨慢性应激抑郁大鼠模型心脏胆碱乙酰转移酶(GAP-43)、酪氨酸羟化酶(TH)表达、心电图表现及心房、心室局部动作电位时程的变化特点。方法采用随机数字表法将20只SD大鼠随机分为抑郁模型组和健康对照组,每组各10只。对抑郁组大鼠进行连续21 d的慢性应激,应用心脏电生理仪及激光共聚焦镜观察大鼠的体表心电图、局部动作电位时程改变及心脏局部组织的GAP43、TH染色分布。结果抑郁组心电图P波较对照组明显延长[(35.09±3.23)ms比(25.43±1.27)ms,P<0.05];抑郁组左、右心房局部动作电位时程均长于对照组[(125.09±6.22)ms比(92.59±7.61)ms,(189.89±14.68)ms比(105.18±15.94)ms,均为P<0.05];抑郁组大鼠心肌GAP43和TH[(22.10±2.42)个/200倍视野,(30.10±1.59)个/200倍视野]分布多于对照组[(13.70±1.82)个/200倍视野,(23.60±2.22)个/200倍视野,均为P<0.01]。结论慢性应激抑郁大鼠模型心脏局部组织GAP-43、TH增多,致心脏电生理紊乱,使心肌组织复极异质性增加,可能介导了慢性应激抑郁大鼠模型心律失常的交感神经重构。Objective To investigate the change of heart tissue action potential and sympathetic nerve regeneration in chronic stress rat model. Methods A total of 20 male Sprague-Dawley rats were randomly divided into depression model group （n = 10） and control group （n=10）. Chronic unpredicted mild stress rat model of depression were made by Cronli methods. Electrocardiogram （ECG） and action potentials of atrial and ventricular local tissue were recorded by Lead-2000 electro-physiological instrument. Choline acetyhransferase （GAP-43） and tyrosine hydroxylase （TH） were measured by confocal laser scanning microscope （LSCM）. Results P wave duration on ECG in depression model group was significantly longer than in control group [ （ 35.09 ± 3.23 ） ms vs. （ 25.43 ± 1.27 ） ms, P 〈 0. 05 ]. Compared to control group, action potential duration of local atrial tissue in depression model group was significantly increased [ （125.09 ± 6. 22） ms vs. （92. 59 ±7.61 ） ms in left atrium, （189. 89 ± 14. 68） ms vs. （105.18± 15.94） ms in right atrium, both P 〈0. 05 ]. There were significantly higher counts of GAP-43 and TH in depression model group than in control group [ （22. 10 ±2.42）/200 times vision vs. （ 13.70 ± 1.82）/200 times vision for GAP-43, and （30. 10 ±1.59）/200 times vision vs. （23.60 ±2. 22）/200 times vision for TH, both P 〈 0. 01 ]. Conclusions GAP-43 and TH is increased, and action potential duration of local heart tissue is prolonged in chronic unpredicted mild stress rat model.

关 键 词：应激 抑郁 电生理学 神经再生 

分 类 号：R749.4[医药卫生—神经病学与精神病学]