原子力显微镜对华蟾素作用的SGC-7901细胞的凋亡研究  

Analysis of Gastric Cancer Cell Line SGC-7901 Influenced by Cinobufacini Using AFM

作　　者：马丽娜[1] 张灵敏[2] 金花[1] 蔡继业[1] 

机构地区：[1]暨南大学化学系,广州510632 [2]暨南大学生物医学工程系,广州510632

出　　处：《肿瘤防治研究》2011年第12期1370-1374,共5页Cancer Research on Prevention and Treatment

基　　金：国家重点基础研究发展计划(973计划)资助项目(2010CB833603);国家自然科学基金资助项目(30872404)

摘　　要：目的应用原子力显微镜(AFM)研究华蟾素对胃癌SGC-7901细胞的作用,在单细胞水平上分析胃癌SGC-7901细胞超微机构的变化,为进一步探讨华蟾素对胃癌SGC-7901细胞的作用机制奠定基础。方法利用原子力显微镜高分辨率、高灵敏度的特点,观察不同浓度(0、6.25、12.5、25、37.5、50ug/ml)华蟾素对胃癌细胞的作用,以及同等浓度药物作用下,随着作用时间(24h,48h,72h,96h)的延长,观察细胞超微结构的变化。同时,利用流式细胞仪对凋亡情况进行分析。结果药物作用后,细胞开始塌陷变矮。细胞表面结构的几何参数,包括高低差(Rp-v)、均方根粗糙度(Rq)、平均粗糙度(Ra)、平均高度(Meant Ht)均出现明显变化,且加药后的细胞凋亡率明显高于对照组(5.04±1.11)%。结论通过AFM观察细胞表面超微结构的变化,从纳米尺度上研究了华蟾素对胃癌细胞的作用,从另一层面增加了对胃癌细胞的认识,从而使AFM有望成为临床肿瘤诊断的一种新仪器。Objective To characterize the morphology of gastric cancer cell line SGC-7901 influenced by Cinobufacini,which is one of the traditional Chinese medicine reagents.The ultrastructure changes of gastric cancer cell line SGC-7901 interacted by Cinobufacini were analyzed by atomic force microscope（AFM） at single-cell level.Method The ultrastructure changes of gastric cancer cell line SGC-7901 interacted by Cinobfacini were analyzed by atomic force microscope（AFM） at single-cell level.And cell apoptotic rate was determined by flow cytometry.Result Compared to the untreated cell,the size of cells decreased,and the nucleolus crimpled.The cellular membrane ultrastructure,Rp-v,Rq,and Ra value were significantly changed.And flow cytometry data indicated that cell apoptosis rate increased with increasing concentration of Cinobufacini.Conclusion AFM-based experiments which helped us increase the awareness of gastric cancer cell line SGC-7901 at visualization will contribute to shedding new light into the structure-function relationships of cell surfaces at single cell or nanometer level.In other words,AFM could be a new useful tool to diagnose cancer at single cell level.

关 键 词：原子力显微镜 华蟾素 胃癌细胞系SGC-7901 超微结构 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]