新型维甲酸衍生物ATPR体外诱导消化系统肿瘤细胞分化的研究  

Effects of 4-amino-2-trifluoromethyl-phenyl Retinate on Differentiation of Human Digestive System Tumor Cells in vitro

作　　者：洪凡青[1] 陈飞虎[1] 吴菲[1] 陈慧慧[1] 

机构地区：[1]安徽医科大学药学院,合肥230032

出　　处：《肿瘤防治研究》2011年第12期1375-1379,共5页Cancer Research on Prevention and Treatment

摘　　要：目的观察新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(ATPR)对胃癌细胞株SGC-7901、肝癌细胞株BEL-7402、结肠癌细胞株HT-29的生长和分化的影响。方法采用四甲基偶氮唑盐(MTT)法,检测细胞的生长抑制率;分光光度法检测胃癌细胞分化标志酶碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性和肝癌细胞分化标志酶LDH、谷酰转肽酶(γ-GT)活性;酶联免疫法检测肝癌细胞标志物甲胎蛋白(AFP)和结肠癌细胞标志物癌胚抗原(CEA)水平;流式细胞仪测定细胞周期的变化。结果 ATPR呈浓度依赖性抑制肿瘤细胞增殖;并使SGC-7901中ALP、LDH活性下降,BEL-7402中AFP、LDH、γ-GT水平下降,HT-29中CEA水平升高;G0/G1期细胞表达量增加,细胞周期进程受影响,细胞阻滞在G0/G1期。结论 ATPR对上述三种实体瘤细胞株具有诱导分化的作用。Objective To study the effect of 4-amino-2-trifluoromethyl-phenyl retinate（ATPR） on the proliferation and differentiation of human gastric cancer cells SGC-7901,human liver cancer cells Bel-7402 and human colon carcinoma cells HT-29.Methods Solid tumor cells were cultured in vitro and treated with ATPR of different concentration.The proliferation was detected with MTT test.ALP,LDH and γ-GT were measured by spectrophotometer for enzyme activity,AFP and CEA were measured by ELISA.The cell cycle was analyzed by flow cytometry.Results The growth of solid tumor cells treated with ATPR was inhibited in a does-dependent manner.ALP and LDH activity of SGC-7901 suppressant.AFP,LDH and γ-GT of Bel-7402 decreased while the expression of CEA of HT-29 increased.G0/G1-phase cells significantly increased while S-phase cells decreased with the elevation of ATPR concentration.Cell cycle progression was blocked in the G1 phase.Conclusion ATPR could induce differentiation of solid tumor cells.

关 键 词：ATPR 实体瘤细胞 增殖 诱导分化 

分 类 号：R735.2[医药卫生—肿瘤] R735.3[医药卫生—临床医学]