枯草芽胞杆菌B-FS01对葡萄霜霉病的防治效果  

Efficacy of the strain B-FS01 of Bacillus subtilis in suppression of the grape downy mildew caused by Plasmopara viticola

作　　者：陈浩[1,2] 胡梁斌[3] 唐春平 胡健[1] 石志琦 

机构地区：[1]扬州大学环境科学与工程学院,扬州225009 [2]江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地农业部食品安全监控重点开放实验室,南京210014 [3]河南科技学院食品学院,新乡453003

出　　处：《植物保护》2011年第6期194-197,共4页Plant Protection

基　　金：江苏省农业科技自主创新资金项目(CX(09)631)

摘　　要：拮抗菌B-FS01具有广谱的拮抗活性,经鉴定为枯草芽胞杆菌,可产生脂肽类抗菌物质fengycins和surfac-tins。室内试验表明,B-FS01发酵液菌体量2.6×107个/mL及其无菌滤液对葡萄霜霉病菌均具有较好的抑制作用,抑制率分别达到了82.48%和68.66%。开发了B-FS01可湿性粉剂,田间试验表明B-FS01可湿性粉剂稀释液菌体量107个/mL对葡萄霜霉病采用保护和治疗两种施药方式,使用3次药后防效分别为88.25%和80.88%,优于对照药剂72%霜脲氰.锰锌可湿性粉剂600倍稀释液。本研究表明B-FS01可湿性粉剂对葡萄霜霉病具有良好的田间应用前景。A broad-spectrum antagonistic strain B-FS01 of Bacillus subtilis was known to produce antimicrobial lipopeptides, including fengycins and surfactins. The inhibition rates of β-FS01 2. 6 × 10^7 spores/mL and its sterile filtrate against Plasrnopara viticola were 82.48% and 68. 66%, respectively in the laboratory. The wettable powder preparation of B-FS01 WP was used in field experiments. The results showed that the biocontrol efficacy of B-FS01 WP diluent 10^7 spores/mL used in preventive and curative experiments for grape downy mildew were 88. 25% and 80. 88%, respectively, after treatment for 3 times. The efficacy value was higher than the chemical fungicide 72% cymoxanil and mancozeb WP （diluted 600 times）. This study suggested that B-FS01 WP had a valuable perspective for controlling the grape downy mildew in fields.

关 键 词：拮抗菌 葡萄霜霉病菌 田间药效 抗菌物质 枯草芽胞杆菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治]