土壤浸提液中重金属对发光杆菌T_3的毒性效应  

Toxicity Effects of Extraction Solutions of Soil Doped with Heavy Metals to Photobacterium phosphoreum T_3

作　　者：李兴春 信晶[2] 杜显元[2] 李鱼[2] 

机构地区：[1]中国石油安全环保技术研究院,北京100083 [2]华北电力大学能源与环境研究院,北京102206

出　　处：《安徽农业科学》2011年第35期21986-21989,共4页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"科技支撑项目(2008BAC43B01)

摘　　要：通过测定掺杂Pb2+、Zn2+、Cd2+、Cu2+和Cr3+的土壤浸提液中明亮发光杆菌T3的抑制率,研究重金属复合污染对明亮发光杆菌T3的生物毒性效应。按照重金属离子等效应浓度配比的原理,设计均匀试验,用逐步多元回归法构建土壤水浸提液中重金属(Pb2+、Zn2+、Cd2+、Cu2+和Cr3+)浓度(Xi)与发光菌抑制率(Y)的响应关系:Y=(-39.596±15.811)-(6.633±0.802)×(-lgXCu)(-lgXZn)+(59.466±8.731)×(-lgXCd)(-lgXCr)。该模型具有较好的拟合优度(F检验和t检验的P<0.05),模型校正后的决定系数(R2adj)为0.933。内部样本验证表明,发光菌抑制率的预测值与试验值相对偏差最大为8.6%,说明该模型具有较好的预测能力。机理分析显示,Cu2+和Zn2+交互体现为拮抗作用,Cd2+和Cr3+交互体现为协同作用,故在重金属复合污染控制方面,有必要进一步研究Cu2+和Zn2+拮抗作用对降低复合毒性的贡献。Toxicity effects of composite pollution of heavy metals to Photobacterium phosphoreum were studied through determining the inhibition rate of Photobacterium phosphoreum in the extraction solutions of soil doped with Pb2＋,Zn2＋,Cd2＋,Cu2＋ and Cr3＋.The response relation [Y=（-39.596±15.811）-（6.633±0.802）×（-lgXCu）（-lgXZn） ＋（59.466±8.731）×（-lgXCd）（-lgXCr）] was developed between concentration of Pb2＋,Zn2＋,Cd2＋,Cu2＋ and Cr3＋ in extraction solutions of soil and the inhibition rate of Photobacterium phosphoreum based on the uniform experimental design,with the principle of equivalent-effect concentration ratio method and the multiple stepwise regression.The model had significant goodness-of-fit with advantages in F-test and t-test（P0.05） and a high adjusted correlation coefficient（R2adj=0.933）.The results in the internal sample validation indicated that the maximum of relative deviation between experimental and calculated values of the inhibition rate was 8.6%.The mechanism analysis showed that the interaction between Cu2＋ and Zn2＋ was antagonistic effect;while the interaction between Cd2＋ and Cr3＋ was synergistic effect.The findings of this study underlined the necessity of further research on the antagonistic effect between Cu2＋ and Zn2＋ to reduce composite toxicity in heavy metal pollution control.

关 键 词：重金属 复合污染 明亮发光杆菌T3 生物毒性 土壤浸提液 

分 类 号：X131.3[环境科学与工程—环境科学]