短芽孢杆菌高产短杆菌素的分段供氧发酵条件优化  

The Optimization of Two Stages with Different Oxygen Supplement for the Production of Tyrothricin from Bacillus Brevis

作　　者：任倩然[1] 张宇光[1] 姜鹭[1] 金君华[1] 任发政[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院教育部功能乳品重点实验室,北京100083

出　　处：《中国奶牛》2011年第22期55-58,共4页China Dairy Cattle

基　　金：十二五国家科技支撑计划项目资助(2011AA100903)

摘　　要：本研究通过优化溶氧条件,对短芽孢杆菌(Bacillus Brevis)(ATCC 8185)进行分段培养,提高发酵液中短杆菌素产量。以析因试验确定菌体生成阶段培养条件为装液量35mL/100mL,摇床转速120r/min;以二次饱和D-最优设计试验确定短杆菌素合成阶段培养条件为:培养36h后静置培养。通过对溶氧条件的优化,发酵液中短杆菌素的产量达到616.93±9.72μg/mL,比优化前提高了89%。This study analyzed the influence of dissolved oxygen on the yield of tyrothricin in flask culture. In order to improve the production of tyrothricin, two stages with different oxygen supplement were applied in the fermentation process of Bacillus Brevis （ATCC 8185）. The culture condition of growth stage were determined by factorial experiment as follows： liquid volume 35mL/ 100mL, rotate speed 120r/min. Through Second Saturating D-Optimal Design, the condition for synthesis stage was set as adjusting to stationary state at 36h of fermentation.After the optimization of dissolve oxygen in flask fermentation, the yield of tyrothricin was 616.93±9.72μg/mL and increased by 89% compared with previous one.

关 键 词：短芽孢杆菌 短杆菌素 溶氧 优化 分段培养 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]