大孔树脂在分离提取灵芝酸的应用  

Application of Macroporous Resin in Extraction and Separation of Ganodenic Acid in Fermentation Solution of Ganoderma Lucidum

作　　者：金德宽[1] 

机构地区：[1]江苏食品职业技术学院食品与营养工程学院,淮安223003

出　　处：《食品工业》2011年第12期21-25,共5页The Food Industry

基　　金：淮安市科技支撑计划项目(HAG20100044)

摘　　要：以灵芝发酵液为原料,通过AB-8大孔树脂动态吸附试验和正交试验的分析,得出的AB-8大孔树脂对灵芝三萜类吸附最佳条件为pH4,体积流量为2BV/h,上样初始质量浓度为0.203mg/mL,上样量为50mL,此时AB-8吸附量达0.408mg/g。并确定最佳洗脱方案:水→体积分数20%乙醇→体积分数40%乙醇→体积分数95%乙醇。The conditions ofAB-8 macroporous resin adsorbing triterpenes in fermentation solution of ganoderma lucidum were optimized by dynamic adsorption experiments and orthogonal experiments. The results showed that adsorption capacity achieved 0.408 mg/g under volume flow at 2 BVha, pH4, sample initial concentration at 0.203 mg/mL and sample volume at 50 mL. The optimal elution could be carried out by using water, 20% ethanol in volume fraction, 40% ethanol in volume fraction, 95% ethanol in volume fraction respectively and sequentially.

关 键 词：大孔树脂 动态吸附 灵芝三萜 

分 类 号：TQ461[化学工程—制药化工]