多烯磷脂酰胆碱注射液对大鼠局灶性脑缺血的保护作用  

The protection of polyene phosphatidylcholine injection in treatment of focal cerebral ischemia in SD rats

作　　者：殷彦[1] 聂亚雄[1] 朱云龙[1] 尹剑[1] 

机构地区：[1]南华大学附属第一医院神经内科,衡阳421001

出　　处：《中国实用神经疾病杂志》2011年第23期10-12,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的研究多烯磷脂酰胆碱注射液对局造性脑缺血大鼠的保护作用。方法若干只SD雄性大鼠,参照Longa法略加修改制作大脑中动脉闭塞模型,并进行神经功能评分,将神经功能评分为2分的18只脑缺血模型的大鼠随机分为3组,即模型组、早期药物干预组、晚期药物干预组,每组6只。药物干预组分别于术后2h、术后第3天开始连续3d腹腔注射多烯磷脂酰胆碱注射液80mg/(kg.d),各组未使用药物时分别给予等量生理盐水注射。造模后第6天清晨再次进行神经功能评分,并采血分离血清,用酶联免疫吸附测定法(ELISA)检测血清中NSE和TNF-α含量。结果用药前3组神经功能评分差异无统计学意义(P>0.05);血清NSE含量和TNF-α含量比较,早期药物干预组、晚期药物干预组与模型组间,差异有统计学意义(P<0.05),但早期药物干预组与晚期药物干预组比较,差异无统计学意义(P>0.05)。结论多烯磷脂酰胆碱注射液能通过减轻炎症反应,对缺血性脑损伤有一定的保护作用。Objective To investigate whether polyene phosphatidylcholine injection has the protective effects on rats with focal cerebral ischemia.Methods According to the method of Longa＇s,with a little change,the animal models were made.Eighteen male SD rats with neurological deficit graded 2 were randomly divided into 3 groups（n=6）： operated group,early-stage administered group,and lated-stage administered group.The last two groups were intraperitoneal injected into the polyene phosphatidylcholine injection after the two hours or the third day of operation at a dosage of 80 mg every kilogram continuous three days,and the same amount of the saline were injected into the three groups when they didn＇t inject drug.After the 6th day of operation,neurological deficit,the content of NSE and TNF-α in serum were measured by enzyme-linked immunosorbant assay（ELISA）.Results Compared with neurological impairment,there were no statistical differences between each other in the three groups（P0.05）,the two groups with drug had lower the content of NSE and TNF-α in serum than the operated groups（P0.05）.Conclusion Polyene phosphatidylcholine injection could play a neuro-protective effect after focal cerebral ischemia through alleviating inflammation.

关 键 词：多烯磷脂酰胆碱注射液 脑缺血 肿瘤坏死因子 神经元特异性烯醇化酶 

分 类 号：R-332[医药卫生]