RT-QPCR法检测早期NSCLC患者外周血中CK-19mRNA的意义  

Using RT-QPCR to detect the expression of CK-19 mRNA in peripheral blood of NSCLC patients

作　　者：刘建[1,3] 任宏轩[1] 伍治平[2] 

机构地区：[1]昆明医学院第三附属医院,云南省肿瘤医院肿瘤化疗中心,云南昆明650118 [2]昆明医学院第三附属医院,云南省肿瘤医院肿瘤研究所,云南昆明650118 [3]盐城市第三人民医院肿瘤科,江苏盐城224000

出　　处：《南京医科大学学报（自然科学版）》2011年第11期1640-1644,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：云南省教育厅科研基金课题(08C0098)

摘　　要：目的:RT-QPCR法探测早期非小细胞肺癌(NSCLC)外周血中CK-19 mRNA的意义。方法:以RT-PCR法从113例NSCLC患者(肺癌组)、50例肺良性病变和50例健康者(对照组)标本中筛选出有CK-19 mRNA表达的标本;再以RT-QPCR法检测表达CK-19 mRNA的标本,通过测定受试者标准曲线下面积(AUC)分析本实验的准确性。结果:①肺癌组和对照组中CK-19mRNA阳性表达率分别为38.94%(44/113)、1.00%(1/100),差异有统计学意义(P<0.01),且肺癌组中CK-19 mRNA的阳性表达与肿瘤分期和分化程度有关;②本实验效果的临界值为0.236,灵敏度为95.45%,准确度为77.27%;AUC为0.896。结论:RT-QPCR法检测外周血中的CK-19 mRNA可作为早期NSCLC患者发生微转移时的一个参考指标,但尚需大样本长期追踪观察证实。Objective: To detect the expression of CK-19 mRNA in peripheral blood of non-small cell lung cancer(NSCLC) patients by real-time fluorescence quantitative polymerase chain reaction(RT-QPCR),and discuss the value of the experiment.Methods: The expression of CK-19 mRNA in peripheral blood of 113 NSCLC patients(NSCLC group),50 non-cancer patients and 50 healthy person(control group) was detected using RT-PCR and found out the positive cases.Then,RT-QPCR was used to detect the expression of relative levels of CK-19 mRNA in positive peripheral blood.The accuracy of this trial was analyzed by receiver operating characteristic curve(ROC curve) and the area under ROC curve(AUC).Results: ① The expression rates of CK-19 mRNA in the NSCLC group and the control group were 38.94%(44/113) and 1.00%(1/100),respectively,there was significant difference between them(P < 0.01),and the expression of CK-19 was related to TNM classification and differentiation of cancer.② The analysis of ROC curve showed that when the point of transition was 0.236,its sensitivity and specificity were 95.45% and 77.27% respectively,and the AUC was 0.896.Conclusion: CK-19 mRNA might be the specific and sensitive marker to detect circulating tumor cells in peripheral blood of NSCLC patients,but it still needs to be proved by further follow-up observation in a large volume sample.

关 键 词：非小细胞肺癌 微转移 细胞角蛋白-19 RT-PCR ROC曲线 

分 类 号：R734.2[医药卫生—肿瘤]