毛细管电泳-电化学发光法分离检测饮料中亮氨酸  

Separation and determination of leucine in beverages by capillary electrophoresis with electrochemiluminesence detection

作　　者：蔡慧农[1] 骆聪婷[1] 王力[1] 

机构地区：[1]集美大学生物工程学院,福建厦门361021

出　　处：《分子科学学报》2011年第6期377-381,共5页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(20871054);福建省教育厅基金资助项目(2007F5076);福建省自然科学基金高校专项基金资助项目(2007J0369)

摘　　要：基于亮氨酸对化学发光试剂三联吡啶钌(Ru(bpy)32+)在铂电极上电致发光信号的增敏作用,建立了一种快速测定亮氨酸的毛细管电泳-电化学发光分析方法.对实验条件进行优化,最佳实验条件如下:检测电位为1.15 V;Ru(bpy)32+浓度为7 mmol.L-1(pH=8.5);进样时间为10 s;进样高压为10 kv;分离高压为14 kv;运行缓冲液pH=8.5,20 mmol.L-1磷酸盐缓冲液.在最佳实验条件下,6 min内可实现亮氨酸的分离检测,线性范围2～320 mg.L-1(R=0.999 8),最低检出限(S/N=3)为0.13 mg.L-1.对饮料样品进行测定,峰高和迁移时间的相对标准偏差(RSD)分别为1.94%和0.50%(n=5).该方法具有分析速度快、分离效果好、灵敏度高、重现性好等特点,适用于饮料中亮氨酸的快速检测.A new sensitive method is described for determination of leucine in beverages by capillary electrophoresis with an electrochemiluminescence detection（CE-ECL） based on tris（2,2′-bipyridyl）ruthenium（Ⅱ） [Ru（bpy）32＋].Experimental parameters influencing the effects of separation and determination were explored in details.Under the optimal conditions,such as detection potential at 1.15 V,7 mmol·L-1 Ru（bpy）32＋（pH=8.5）,sample injection at 10 s×10 kV,separation voltage at 14 kV,20 mmol·L-1 of phosphate buffer at pH=8.5,the determination of leucine was accomplished within 6min.The ECL intensity was linear with the concentration of leucine in the range of 2～320 mg·L-1（R=0.999 8） with the detection limit（S/N=3） of 0.13 mg·L-1.The relative standard deviations（RSD） of ECL intensity and migration time for five injections of beverage sample were 1.94% and 0.50%（n=5）,respectively.This method was successfully used to determination of Leucine in beverage with satisfactory results.

关 键 词：毛细管电泳 电化学发光 三联吡啶钌 亮氨酸 饮料 

分 类 号：O657[理学—分析化学]