血清及房水中VEGF、IL-6水平与新生血管性青光眼的相关性研究  

Study of the correlation between serum,aqueous humor VEGF,IL-6 levels and the neovascular glaucoma

作　　者：李莉[1] 刘升强[1] 王帅[1] 孙时磊[1] 邵彦[1] 王健[1] 王怡[1] 李静敏[1] 

机构地区：[1]大连医科大学附属第二医院眼科,116021

出　　处：《临床眼科杂志》2011年第6期490-493,共4页Journal of Clinical Ophthalmology

摘　　要：目的测定新生血管性青光眼(NVG)患者血清及房水中血管内皮生长因子(VEGF)、白细胞介素-6(IL-6)的水平,探讨VEGF、IL-6在NVG发生发展中的作用。方法选取NVG患者20例(20只眼)作为实验组(A组),原发性慢性闭角型青光眼患者(B组)、老年性白内障患者(C组)各20例(20只眼)作为对照组。采集3组患者血清及房水标本,通过双抗体夹心酶联免疫吸附试验(ELISA法)分别检测血清与房水中VEGF、IL-6的水平。结果 (1)A组房水中VEGF、IL-6的水平(1336.80±70.15)pg/ml、(691.15±50.09)pg/ml明显高于B组(311.60±31.06)pg/ml、(168.25±11.95)pg/ml和C组(165.75±13.95)pg/ml,(92.10±9.59)pg/ml,3组间两两比较,差异均具有统计学意义(F=4019.334,P<0.01;F=2275.019,P<0.01)。A组血清中VEGF、IL-6的水平(545.40±155.49)pg/ml、(291.35±22.66)pg/ml高于B组(321.15±52.57)pg/ml、(104.35±13.21)pg/ml和C组(176.30±20.38)pg/ml、(87.00±12.70)pg/ml,3组间两两比较,差异均具有统计学意义(F=75.940,P<0.01;F=906.947,P<0.01)。(2)A组房水中VEGF与IL-6的水平呈显著的正相关性,差异具有统计学意义(r=0.857,P<0.01)。其余各组标本中无显著的相关性(P>0.05)。结论 NVG患者房水及血清中VEGF、IL-6的水平明显高于对照组,且房水中二者水平呈明显正相关,提示在NVG病理机制过程中,VEGF、IL-6作为促血管生成因子,相互促进、相互影响,共同导致了NVG的发生和发展。Objective This study was designed to discuss the role of VEGF,IL-6 in the development of NVG,by detecting the NVG serum and aqueous humor VEGF and IL-6 levels.Methods 20 patients with NVG were selected as experimental group（group A）,20 patients with primary chronic angle closure glaucoma patients（group B）,and 20 cases of senile cataract patients（group C） as a control group.three specimens of serum and aqueous humor were collected and the serum and aqueous humor VEGF,IL-6 levels were measured through the double-antibody sandwich ELISA（enzyme-linked immuno sorbent assay,ELISA method）.Results（1）The levels of VEGF,IL-6 in aqueous humor： group A（1336.80±70.15） pg / ml,（691.15±50.09） pg/ml was significantly higher than that of group B（311.60±31.06） pg/ml,（168.25±11.95） pg/ml and the group C（165.75±13.95）pg/ml,（92.10±9.59） pg/ml.among the three groups,both the differences of pairwise comparison were significant（F= 4019.334,P0.05;F=2275.019,P0.05）.the levels of serum VEGF,IL-6： group A（545.40±155.49） pg / ml,（291.35 ± 22.66） pg / ml was higher than the group B（321.15±52.57） pg/ml,（104.35±13.21） pg / ml and the group C（176.30±20.38） pg / ml,（87.00±12.70） pg / ml,pairwise comparison among the three groups,the difference was statistically significant（F=75.940,P0.05;F=906.947,P0.05）.）（2）NVG group of aqueous VEGF and IL-6 concentration showed a significant positive correlation and statistically significant difference（r=0.857,P0.05）.The remaining specimens in each group showed no significant correlation（P0.05）.Conclusion The VEGF,IL-6 levels in the aqueous and serum of patients with NVG were significantly higher than those for the other two groups,and the correlation between the two levels in the aqueous humor was significant positive,suggesting that the VEGF,IL-6 as an angiogenic factor,promotted and influenced in mutual,in the process of NVG pathogenesis,together led to the occurrence and development of the NVG.

关 键 词：新生血管性青光眼 血管内皮生长因子 白细胞介素6 

分 类 号：R775[医药卫生—眼科]