不同巢材鸟巢中优势菌群的分离鉴定  

Isolation and Identification of Dominant Bacterial Population from Different Bird Nest Materials

作　　者：朱春玉[1] 徐剑[1] 万冬梅[1] 李辉[1] 霍雅鹏[1] 

机构地区：[1]辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室,辽宁沈阳110036

出　　处：《微生物学杂志》2011年第5期14-19,共6页Journal of Microbiology

基　　金：国家自然科学基金(30670288;31071927);辽宁省教育厅重点实验室项目(2008S099)

摘　　要：从鸟类苔藓和草叶巢材中分离出6株优势细菌和8株放线菌,采用传统表观型特征观察和16S rRNA基因序列分析法对其进行菌种鉴定。结果表明:草叶巢材中的优势细菌分别为霍氏肠杆菌(Enterobacter hor-maechei)、麝香石竹假单胞菌(Pseudomonas caryophylli)和丁香假单胞菌(Pseudomonas syringae);苔藓巢材中的优势细菌分别为苏云金芽胞杆菌(Bacillus thuringiensis)、阴沟肠杆菌(Enterobacter cloacae)和马氏棒杆菌(Corynebacterium matruchotii);从2种巢材中分离出的8株放线菌均为链霉菌属(Streptomyces),其中4株分别为金色链霉菌(Streptomyces aureus)、红色淡紫灰链霉菌(Streptomyces rubrolavendulae)、胶样链霉菌(Streptomycesgelaticus)和灰色链霉菌(Streptomyces griseus),另外4株链霉菌有待于进一步鉴定。Six dominant bacterial strains and eight actinomycctes strains were isolated from moss and grass/leaves bird nest materials （BNM） and identified through general classification methods including colony characteristics, physiological and biochemical properties etc. and 16S rRNA gent sequence analyses. The results showed that the dominant bacterial strains from grass/leaves BNM were determined as Enterobacter hormaechei, and Pseudomonas caryophylli, Pseudomonas syringae respectively ; the dominant bacterial strains from moss BNM were Bacillus thuringiensis, Enterobacter cloacae, and Corynebacterium matruchotii respectively. In addition, aetinomyeetes strains isolated from two BNM were Streptomyces. Of which four strains were Streptomyces aureus, Streptomyces rubrolavendulae, Streptomyces gelaticus, and Streptomyces griseus. Another four remained to be identified further.

关 键 词：鸟类巢材 优势细菌 苔藓 

分 类 号：Q93-31[生物学—微生物学]