凝胶电泳与激光剥蚀-电感耦合等离子体质谱联用测定蛋白质中微量元素的应用进展  

Progress in Combination of Gel Electrophoresis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Trace Elements Determination in Proteins

作　　者：王颖[1] 郭艳丽[1,2] 袁洪林[1] 魏永锋[2] 闫宏涛[2] 陈慧慧[2] 

机构地区：[1]大陆动力学国家重点实验室,西北大学地质学系,陕西西安710069 [2]西北大学合成与天然功能分子化学教育部重点实验室,西北大学化学与材料科学学院,陕西西安710069

出　　处：《光谱学与光谱分析》2012年第1期223-228,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(40973011);陕西省自然科学基金资助项目(2010JM2022);西北大学大陆动力学国家重点实验室科技部专项经费项目(BJ11070)资助

摘　　要：介绍了凝胶电泳(GE)和激光剥蚀-电感耦合等离子体质谱(LA-ICP-MS)联用测定蛋白质中微量元素的方法,对蛋白质分离、凝胶处理,包括染色及干燥,和检测过程中的定量校正等技术问题做了详述,并综述了LA-ICP-MS在硒蛋白、磷酸化蛋白及金属蛋白分析中的应用,指出了这种方法在蛋白质中微量元素检测中存在的问题和发展方向。Laser ablation inductively coupled plasma mass s pectrometry（LA-ICP-MS） has become a very efficient and sensitive trace,ultra tr ace,and surface analytical technique for the in situ study of the concentration and distribution of the elements in life sciences with high spatial resolution. It is being used more and more frequently in biological,medical materials and protein research,which will lead to a better understanding of physiology and pa thology process in cells and tissues.The present review mainly introduces the s trategies of combination of gel electrophoresis（GE） with LA-ICP-MS for the qu an tification of trace elements in proteins,including the proteins separation,ele ments detection and calibration methods.The paper emphasizes the basic conditio ns of the proteins separation,focusing on the stability of proteins during GE a nd the treatment methods of staining and drying of the gel to enable successful detection of the elements by LA-ICP-MS.In addition,the application of GE-LA-ICP-MS in phosphoproteins,selenoproteins and metal-binding proteins is intr oduced in detail.The prospects and challenge for this technique are discussed as well for further study.

关 键 词：激光剥蚀-电感耦合等离子体质谱(LA-ICP-MS) 凝胶电泳(GE) 硒蛋白 磷酸化蛋白 金属蛋白 微量元素 

分 类 号：O657.6[理学—分析化学]