棓丙酯对红细胞溶血和渗透脆性的影响  

Propyl Gallate on Hemolysis and Osmotic Fragility of Red Blood Cells

作　　者：蔡燕[1,2,3,4] 蒋红[2] 彭乙华[5] 邢艳[1,2,3,4] 雷佳红[1,2,3,4] 杨明辉[1,2,3,4] 

机构地区：[1]川北医学院附属医院检验科 [2]川北医学院附属医院风湿免疫研究所 [3]川北医学院附属医院 [4]川北医学院检验系,四川南充637007 [5]川北医学院附属医院内分泌科,四川南充637000

出　　处：《临床医学工程》2011年第12期1852-1853,共2页Clinical Medicine & Engineering

基　　金：四川省卫生厅科研项目(编号:100165)

摘　　要：目的探讨棓丙酯是否可影响红细胞溶血及其渗透脆性。方法以不同浓度的棓丙酯(256μg/ml、128μg/ml、64μg/ml、32μg/ml、16μg/ml、8μg/ml)与红细胞悬液孵育12小时,肉眼观察和氰化高铁血红蛋白法测定溶血程度;红细胞渗透脆性试验检测药物处理后各组红细胞的最大脆性和最小脆性,再用氰化高棓铁血红蛋白法检测各组的最大脆性溶血情况。并用统计软件SPSS16.0对实验结果进行统计分析。结果肉眼观察和氰化高铁血红蛋白法显示不同浓度的棓丙酯处理后,各组红细胞悬液无明显溶血,各药物处理组的最大脆性和最小脆性与正常人和对照组的渗透脆性比较无显著差异;氰化高铁血红蛋白法检测各处理组的最大脆性溶血程度与对照组比较差异无显著性(P>0.05)。结论棓丙酯对红细胞溶血和渗透脆性无影响。Objective To research whether the propyl gallate can influence the hemolysis and osmotic fragility of red blood cells. Methods Different concentrations of propyl gallate （ 256 μg/ml, 128 μg/ml, 64 μg/ml, 32 μg/ml, 16 μg/ml, 8 μg/ml） were incubated with red blood cells for 12 hours. The levels of hemolysis were detected by visual observation and hemiglobincyanide; The red blood cell osmotic fragility test was used to detected the maximum and minimum osmotic fragility. The hemiglobincyanide was also used to detect the hemolytic status of maximum osmotic fragility. The statistical software SPSS 16.0 was applied to analyze the results. Results No significant hemolysis was observed by eyes. There were no conspicuous differences of the maximum and minimum osmotic fragility among the experimental groups, the control group and the reference value of health adults. When comparing the hemolysis of the the maximum osmotic fragility, there were no significant differences （P 0.05）. Conclusion Propyl gallate can＇t influence the hemolysis and osmotic fragility of red blood cells.

关 键 词：棓丙酯 红细胞 渗透脆性 氰化高铁血红蛋白 

分 类 号：R735.7[医药卫生—肿瘤] R730.44[医药卫生—临床医学]