微生物酵素对抗生素相关性肠黏膜屏障损伤大鼠的保护作用  

Protective effects of microbial ferment on intestinal barrier in rats with antibiotic-associated injury

作　　者：孙莉波[1] 李宁[1] 张海涛[1] 周京安[1] 陈涛[1] 刘秀红[1] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069

出　　处：《中华实验和临床感染病杂志（电子版）》2011年第4期5-9,共5页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的观察应用微生物酵素对大鼠抗生素(克林霉素磷酸酯)相关性肠黏膜屏障损伤的保护作用。方法制备大鼠抗生素相关性腹泻模型,分别对抗生素相关性腹泻组与微生物酵素喂养组模型的下腔静脉血行D-乳酸和二胺氧化酶(DAO)测定、细菌培养以及肝脏组织和肠系膜淋巴结行组织细菌培养、回肠内容物菌群培养、回肠末端行病理检查并评分,将两组数据进行统计学分析。结果与单用抗生素组相比,微生物酵素喂养组大鼠于第7天时血浆中DAO和D-乳酸水平显著降低(P<0.05),肠内容物中大肠埃希菌(7d,P<0.01)和肠球菌(7d,P<0.05)显著减少,乳酸杆菌(5d,P<0.05;7d,P<0.01)和双歧杆菌(5d、7d,P<0.01)显著增多,7d时肝脏和肠系膜淋巴结的培养阳性率显著降低(P<0.05),下腔静脉血细菌培养阳性率无显著差异,7d时回肠病理评分显著降低(P<0.01)。结论应用抗生素(克林霉素磷酸酯)可以造成肠道菌群紊乱及肠黏膜屏障损害,导致肠道黏膜上皮通透性升高,菌群易位以及肠外器官的损伤。加用微生物酵素可加强肠黏膜屏障,降低肠道的通透性,减少菌群易位,预防肠源性感染的发生。Objective To observe the antibiotic（clindamycin phosphate） induced injury of intestinal barrier and the protection of microbial ferment on antibiotic-associated injury of intestinal barrier in rats.Methods Total of 63 male healthy SD rats were randomly distributed into 3 different groups：blank control（BC group）,antibiotic-induced injury group（AB group）,antibiotic-induced injury and protection of microbial ferment group（AB ＋ BF group）.AB group and AB＋BF group were divided into l,3,5,7 days groups,respectively.Rats in AB group and AB＋BF group were injected with Clindamycin Phosphate 120 mg/kg subcutaneously,twice per day in the following 7 days,and the AB ＋ BF group were feeded with l g microbial ferment,twice per day in the following 7 days.The serum concentrations of D-lactic and diamine oxidase（DAO） were measured at l,3,5,7 days,respectively.The microbial floras in ileum were cultivated and counted.Liver tissue,mesenterium lymphaden and blood were cultivated.The pathological changes of terminal ileum tissue were observed.SPSS 11.5 software was used for statistical analysis.Results Compared with AB group,indexes in AB ＋ BF group were as following：the serum concentrations of DAO and D-lactic were significantly lower（P〈0.05）,Escherichia coli（7 days,P〈0.01） and Enterococci（7 days,P〈0.05） in ileum significantly decreased,Bacillus acidi lactici（5 days,P〈0.05;7 days,P〈0.01） and Bacillus bifidus（5 days,7 days,P〈0.01） increased,the cultivation positive rates of liver and mesenteric lymphonodi decreased（7 days,P〈0.05）,the cultivation positive rates were not significantly different,the pathological scores of terminal ileum tissue were much lower（7 days,P〈0.01）.Conclusions Long period（at least 5-7days） application of antibiotics（clindamycin phosphate） could induce disorder of microbial floras and injury of intestinal barrier,increase intestinal tract permeability,cause bacterium translocation and another organ inflammatory injury.The appli

关 键 词：微生物酵素 抗菌药 菌群失调 二胺氧化酶 D-乳酸 

分 类 号：R965[医药卫生—药理学]