年龄相关性白内障患者房水中纳米细菌的培养和检测  

Cultivation and identification of nanobacteria in aqueous humor of patients with age-related cataract

作　　者：郑幼平[1] 蓝兴锐[2] 郭少琼[2] 刘洁梅[1] 王桂芳[3] 

机构地区：[1]中国广东省广州市番禺区中医院眼科,511400 [2]中国广东省广州市番禺区中医院检验科,511400 [3]暨南大学第一附属医院眼科,中国广东省广州市510630

出　　处：《国际眼科杂志》2012年第1期98-100,共3页International Eye Science

基　　金：中国广州市番禺区科技攻关计划资助项目(No.2009-Z-123-1)~~

摘　　要：目的:分离、培养与检测年龄相关性白内障房水中的纳米细菌(nanobacteria,NB),为进一步探讨NB致白内障的机制奠定基础。方法:白内障超声乳化联合人工晶状体植入手术术前收集30份房水标本,通过脱矿、过滤、离心处理,用细胞培养的方法进行NB培养,观察其生长情况。采用免疫组织化学、透射电镜方法观察培养物形态。结果:培养6wk后,对培养标本进行观察,发现12瓶(40%)培养液中存在絮状漂浮成团的NB,并且其中8瓶培养液瓶底亦可见贴壁生长的成团的或散在分布的颗粒沉淀状NB;对照组一看见1例絮状漂浮,并可见管底颗粒沉淀;对照组二未见絮状漂浮和颗粒沉淀。透射电镜可见NB为针状物的聚集体,大小不一。结论:从年龄相关性白内障患者房水中分离培养鉴定出NB,表明其感染与白内障发病可能有关,需进一步研究其致病机制。AIM：To isolate,culture and identify the nanobacteria（NB） from aqueous humor of patients with age-related cataract in our department.METHODS：Sample nanobacteria in aqueous humor of 30 patients with age-related cataract were collected for NB culture in this study.Immunohistochemistry,transmission electron microscopy（TEM） were used to investigate the appearance and components of cultured NB.RESULTS：Twelve aqueous samples out of 30（40%） showed growth of NB,1 of 5 showed growth of NB in the saline control group,but none growth in the control group.TEM showed needle-like nanobacteria aggregates of different sizes.CONCLUSION：For the first time we isolated,cultured and identified nanobacteria in aqueous of patients with age-related cataract,indicating that nanobacteria infection is related with age-related cataract.

关 键 词：年龄相关性白内障 纳米细菌 感染 房水 

分 类 号：R776.1[医药卫生—眼科]