细胞外基质蛋白-1与基质金属蛋白酶-9在肝细胞癌中的表达及其临床意义  

Expressions of extracellular matrix protein 1 and matrix metalloproteinase-9 in hepatocellular carcinoma and their clinical significances

作　　者：荚卫东 陈浩 李建生 许戈良 马金良 王伟[1] 葛勇胜 余继海 

机构地区：[1]安徽医科大学附属省立医院肝癌研究中心,安徽医科大学附属省立医院肝脏外科,合肥230001

出　　处：《中华肝胆外科杂志》2011年第12期989-993,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：基金项目：国家自然科学基金项目（30772097）;安徽省高等学校省级自然科学研究项目（KJ20108386）;安徽省自然科学基金项目（070413073）;安徽省优秀青年科技基金项目（08040106818）;安徽省“115”产业创新团队项目

摘　　要：目的 探讨细胞外基质蛋白-1 （ECM1）和基质金属蛋白酶-9（MMP-9）在肝细胞癌组织中的表达及其与肝癌侵袭、转移及预后的关系.方法 采用免疫组织化学法检测120例肝癌组织及17例正常肝组织中ECM1和MMP-9的表达情况.结果 ECM1和MMP-9在肝癌组织中的表达阳性率分别为73.3％和65.0％,明显高于正常肝组织中的23.5％和11.8％.ECM1和MMP-9在肝癌组织中的表达与肿瘤血管侵犯及TNM分级相关,而且MMP-9还与肿瘤的大小、结节数、分化程度和肝硬化程度有关（P＜0.05）.两者均与患者的年龄、性别、AFP水平、HBsAg、Child分级及肿瘤包膜无关（P＞0.05）.ECM1和MMP-9阳性表达患者的总生存率和无瘤生存率明显低于阴性表达患者.ECM1与MMP-9两者在肝癌中的表达显著正相关（r=0.585,P＜0.001）.结论 ECM1和MMP-9的表达与肝癌的侵袭特性相关,与肝癌术后复发密切相关,可作预测肝癌患者手术预后及复发的指标.Objective To investigate the expressions of extracellular matrix protein 1 （ECM1）and matrix metalloproteinase-9 （MMP-9） in hepatocellular carcinoma （HCC） and to explore their correlations with metastasis and recurrence.Methods Immunohistochemisty was employed to determine the expressions of ECM1 and MMP-9 in 120 HCC and 17 normal liver tissues.Results The positive rates of ECM1 and MMP-9 expression in HCC tissues were 73.3% （88/120） and 65.0% （78/120）,respectively.They were significantly higher than that in normal liver tissues.The expression of ECM1 in HCC tissues was significantly correlated with vascular invasion and TNM stage.The expression of MMP-9 in HCC tissues was significantly correlated with tumor size,number of tumor nodules,differentiation of tumor and degree of cirrhosis.They were not correlated with age,gender,serum AFP level,HBsAg,Child-Pugh class and capsulated tumor.Patients with ECM1 and MMP-9 positivity had significantly poorer overall and disease-free survival rates.There was a significant positive correlation between ECM1 and MMP-9 （r=0.585,P〈0.001）.Conclusion Both ECM1 and MMP-9 were significantly related to invasion and metastasis.Over-expression of both ECM1 and MMP-9 had predictive values for prognosis and recurrence of HCC after surgery.

关 键 词：肝细胞癌 细胞外基质蛋白-1 基质金属蛋白酶-9 预后 

分 类 号：R735.7[医药卫生—肿瘤]