马钱子碱纳米微粒抗肝细胞癌作用的实验研究  

Experimental study on the therapeutic effect of brucine nanoparticles on hepatocellular carcinoma

作　　者：秦建民[1] 撒忠秋[1] 徐夏君[1] 杨林[1] 盛霞[2] 张敏[1] 李琦[3] 殷佩浩[1] 高科攀[4] 陈庆华[4] 

机构地区：[1]上海中医药大学附属普陀医院普外科,200062 [2]上海中医药大学附属普陀医院病理科,200062 [3]上海中医药大学附属普陀医院肿瘤科,200062 [4]上海医药工业研究院

出　　处：《中华肝胆外科杂志》2011年第12期1005-1008,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：基金项目：上海市教委（No.07CZ017）和国家自然科学基金（No.30873341）资助

摘　　要：目的 观察马钱子碱纳米微粒的抗肝细胞癌作用.方法 采用超声乳化法研制羧基化聚乙二醇-聚乳酸嵌段共聚物马钱子碱纳米微粒,体外观察其对肝癌细胞SMMC-7721生长的影响；采用Western blotting和FQ-PCR检测药物作用72 h对肝癌细胞凋亡相关蛋白Fas蛋白和mRNA表达水平的影响.结果 马钱子碱纳米微粒平均粒径（146±96） nm,载药率：4.2％,包封率：67％.随药物浓度的增加,肝癌细胞生长抑制作用明显,呈现剂量依赖性.5-FU、马钱子碱和马钱子碱纳米微粒对肝癌细胞生长的IC50分别为16.7 μg/ml、90.3 μg/ml和164.9 μg/ml,三者之间差异显著（P＜0.05）.与空白纳米微粒相比,马钱子碱纳米微粒显著诱导肝癌细胞Fas蛋白和mRNA表达,且320 μg/ml剂量诱导表达显著高于160 μg/ml （P＜0.05）.结论 马钱子碱纳米微粒显著抑制肝癌细胞生长,诱导肝癌细胞凋亡,可能与上调肝癌细胞Fas蛋白和mRNA表达有关.马钱子碱纳米微粒是一种具有潜在发展前景的肝癌治疗药物.Objective To observe the therapeutic effect of brucine nanoparticles on hepatocellular carcinoma.Methods Brucine nanoparticles with block copolymer of carboxylation polyethylene glycol and polylactic acid were manufactured by ultraphonic emulsification.The effect brucine nanoparticles on growth of SMMC-7721 cell line was observed in vitro.The protein and mRNA levels of Fas were measured with Western blotting and FQ-PCR respectively after the brucine nanoparticles were added into cell culture fluid for 72 hours.Results The mean diameter,the carried drug rate and the entrapment rate of brucine nanopaticles was 146±96 nm,4.2% and 67%,respectively.The growth inhibition of liver cancer cells was enhanced significantly with the increasing drug dose.The IC50 of growth inhibition of 5-FU,brucine and brucine nanoparticles was 16.7 μg/ml,90.3 μg/ml and 164.9 μtg/ml,respectively.There was significant difference among them （P〈0.05）.The protein and mRNA expression of Fas in brucine nanoparticles treated SMMC-7721 cells increased significantly compared with that in blank control group（P〈0.05）.Conclusion Brucine nanoparticles may potentially be a novel therapeutic drug for hepatocellular carcinoma.

关 键 词：马钱子碱 纳米微粒 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]