MMP -9和C- Met与星形细胞肿瘤恶性程度及其血管生成之间的关系  

Expression of c -Met and matrix metalloproteinase and their correlation with malignancy and angiogenesis in astrocyte tumors

作　　者：赵光宇[1] 栾立明[1] 刘滨[1] 庞琦[1] 

机构地区：[1]山东大学附属省立医院神经外科,济南250021

出　　处：《中华神经外科杂志》2011年第12期1268-1271,共4页Chinese Journal of Neurosurgery

基　　金：山东省优秀中青年科学家科研奖励基金（博士基金）（2007BS03058）

摘　　要：目的 探讨肝细胞因子受体（C - Met）和基质金属蛋白酶（MMP -9）与星形细胞肿瘤血管生成、病理学分型的关系及C - Met和MMP -9之间的相互关系.方法 应用免疫组化（S-P）方法对93例星形细胞瘤组织中的C - Met、MMP -9和微血管密度（MVD）进行检测.应用实时荧光定量聚合酶链反应（ FQ - PC R）检测C- Met和MMP -9的mRNA表达.结果 93例星形细胞肿瘤中C - Met和MMP -9的蛋白阳性率分别为97.8％和95.9％,且其表达水平与mRNA表达均随星形细胞瘤恶性程度的增高而增强（P＜0.01）.星形细胞瘤的MVD随恶性程度的增高而增高（P＜0.01）,C - Met及MMP -9之间的蛋白及mRNA表达水平存在相关关系（P＜0.01）.结论 C -Met及MMP -9的增强表达与星形细胞瘤恶性程度及肿瘤新生血管生成有密切关系,其表达可作为判断星形细胞瘤患者预后的指标.Objective To study the expression of C - Met and MMP - 9 in low - grade and high -grade astrocytomas and its correlation with angiogenesis,pathological classification and prognosis.Methods The protein expression of C - Met and MMP - 9 was examined with immunohistochemical staining Method （S- P）.The mRNA expression of C- Met and MMP- 9 was examined with RT- PCR.Results The positive rates of C - Met and MMP -9 in 98 astrocyte tumor cells were 97.8% and 95.9%respectively,and the degree of positive cells was in accordance with the malignancy of tumors.The MVD of astrocyte tumors correlated with the malignancy positively.There was correlation between C - Met and MMP - 9.Conclusions The up - regulated expression level of C - Met and MMP - 9 is closely related to the malignancy and angiogenesis of tumors.The expression of protein and mRNA may act as an indicator for the prognosis of patients with astrocyte tumors.

关 键 词：星形细胞瘤 肝细胞生长因子 基质金属蛋白酶 新生血管化 

分 类 号：R730.2[医药卫生—肿瘤]