肾透明细胞癌组织中T细胞淋巴瘤侵袭和转移诱导蛋白1表达及其临床意义  

Expression and clinical significance of T-lymphoma invasion and metastasis 1 protein in renal cell carcinoma

作　　者：孟庆泽[1] 乔保平[1] 宫璀璀 刘德海 张喜卿 李琦[1] 马志远[1] 董新强 

机构地区：[1]郑州大学第一附属医院泌尿外科,450052 [2]解放军第一五三中心医院中心实验室 [3]解放军第一五三中心医院泌尿外科 [4]解放军第一五三中心医院病理科

出　　处：《中华泌尿外科杂志》2011年第12期815-818,共4页Chinese Journal of Urology

基　　金：河南省教育厅基金资助项目（2006320047）

摘　　要：目的探讨肾透明细胞癌组织中T细胞淋巴瘤侵袭和转移诱导蛋白1（T—lymphoma invasion and metastasis 1，Tiaml）的表达与临床病理特征及预后的关系。方法选取原发性肾透明细胞癌组织标本107例。男78例，女29例。年龄32～87岁，平均59岁。肿瘤大小按手术切除标本测量。肿瘤最大径1．4～7．6em，平均4．5cm。≤2．5cm者23例，2．6～5．0cm者65例，〉5．0cm者19例。有淋巴结转移者46例，有血管浸润者32例。Fuhrman分级：Ⅰ级22例，Ⅱ级41例，Ⅲ级27例，Ⅳ级17例。2002AJCCTNM分期：T。29例，T：44例，L16例，T。18例。20例正常。肾组织标本作为对照组。免疫组织化学方法检测标本中Tiaml蛋白表达，结合临床病理学资料进行分析。随访5～8年，生存分析采用Kaplan—Meier法，组间差异采用Log—rank检验。结果20例正常肾组织中，ri—aml蛋白阳性表达率为15．0％（3／20），肿瘤组织中Tiaml蛋白阳性表达率为73．8％（79／107），组间差异有统计学意义（P〈0．01）；Ⅰ～Ⅱ级与Ⅲ～Ⅳ级肾癌组织中Tiaml蛋白高表达阳性率分别为47．6％（30／63）、72．7％（32／44），T1～T2期与T3～T4期肾癌组织分别为49．3％（36／73）、76．5％（26／34），无淋巴结转移组与有淋巴结转移组分别为47．5％（29／61）、71．7％（33／46），无血管浸润组与有血管浸润组分别为48．O％（36／75）、81．3％（26／32），组间差异均有统计学意义（P〈0．01）。Tiaml蛋白高表达组与低表达组患者术后5年生存率分别为46．8％（29／62）、84．4％（38／45），组间差异有统计学意义（P〈0．05）。结论肾癌组织中Tiaml蛋白阳性表达率明显高于正常组织，Tiaml蛋白高表达与肾癌的分期、分级、淋巴结转移、血管浸润有关，与患者性别、年龄、肿瘤大小无相关性。检测Tiaml蛋白有助于。肾癌的诊断及预后评估。Objective To explore the expression of Tiaml in clear cell renal cell carcinoma and analyze its correlations to pathology of disease and prognosis. Methods The expressions of Tiaml protein in 107 specimens of human clear cell renal cell carcinoma and 20 specimens of normal renal tissues were detected by immunohistochemical staining and its clinical significance was then analyzed. Results The expression of Tiaml protein was higher in renal cancers than in the adjacent normal tissues （ P 〈 0.01 ）. Tiaml protein expression rates were 47.6% and 72.7% in Ⅰ- Ⅱ and Ⅲ - Ⅳtumors, while 49.3% and 76.5% in T1 - T2 and T3 - T4 tumors, respectively （ P 〈 0.01 ）. Expression of Tiaml protein was higher in lymph node positive renal carcinoma tissues than in lymph node negative renal carcinoma tissues （71.7% versus 47.5% , P 〈 0.05）. The expression of Tiaml in carcinoma tissues showed a positive relationship with tumor vascular invasion （81.3% versus 48.0% , P 〈0.01 ）. In patients followed-up 5 -8 years, Kaplan-meier analysis and the log-rank test showed that the 5-year survival was significantly different between the group of lower and higher Tiaml expression groups （84.4% versus 46.8% , P 〈 0.05）. Conclusions The expression of Tiaml protein was higher in human primary renal carcinoma than in normal renal tissues. The positive rate of Tiaml protein expression was related to classification, TNM stage, lymph node metastasis and vascular invasion. The detection of the expression of Tiaml protein may be helpful in the diagnosis and prognosis of renal carcinoma.

关 键 词：肾肿瘤 T细胞淋巴瘤侵袭和转移诱导蛋白1 免疫组织化学 

分 类 号：R737.11[医药卫生—肿瘤]