SiRNA干扰LI-cadherin对荷人肝癌裸鼠移植瘤的影响  

The effect of LI-cadherin-SiRNA protein on transplanted human hepatoceilular carcinom in nude mice

作　　者：范正军[1] 程波[1] 乔师师[1] 薛建锋[1] 房祥杰[1] 刘艳杰[1] 

机构地区：[1]郑州大学第一附属医院肝胆外科河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《中华普通外科杂志》2011年第12期1031-1034,共4页Chinese Journal of General Surgery

基　　金：河南省科技厅资助项目（092300410020）

摘　　要：目的 研究肝肠粘连蛋白（liver intestine cadherin,LI-cadherin）在荷瘤裸鼠体内对肝癌细胞Hep3B侵袭转移能力的作用.方法 体外培养肝癌细胞株Hep3B,并进行针对LI-cadherin的SiRNA转染.将Hep3B细胞及转染后的肿瘤细胞接种入裸鼠脾脏,建立裸鼠荷瘤模型.观察成瘤及转移效果,免疫组化检测转移灶组织结构,Western blot方法检测转染前后荷瘤裸鼠体内肿瘤转移灶中LI-cadherin的表达.结果 （1）SiRNA质粒转染入Hep3B肝癌细胞,转染率达到80％.（2）建立裸鼠荷人肝癌模型,30只裸鼠移植手术后均成活,荷瘤率达到60％以上.SiRNA转染组荷瘤率为80％,转移灶个数为26个,明显高于空白对照组和空质粒转染组.（3）裸鼠体内转移灶中Ll-cadherin 的含量SiRNA转染组明显低于空白对照组和空质粒转染组.结论 LI-cadherin对肝癌细胞的黏附能力有重要作用,对LI-cadherin进行SiRNA干扰可增强肝细胞癌在裸鼠体内的侵袭转移能力.Objective To investigate the effect of LI-cadherin- SiRNA protein on the growth and metastatic potentials of transplanted human hepatocellular carcinoma cell lines （Hep3B） in nude mice.Methods We transfected LI-cadherin- SiRNA to Hep3B cells,Hep3B cell suspension （transfected or control ） was injected subcapsullaryly into the spleen of nude mice,hepatic metastasis was observed by naked eye and immunohistochemistry.In addition,Western-blot was used to detect the level of LI-cadherin in different metastatic site.Results （1） Hep3B was green after successful transfect interference vector under fluorescence inverted microscope,in this study,the transfect rate was 80%.（2） Hep3B liver metastasis model in nude mice was established.The metastasis rates in the empty plasmid carrying group,the control group and the SiRNA transfect group were 50%,60% and 80%,respectively.The number of metastasis caner nodules in the SiRNA transfect group was 26,significantly higher than other two groups.（3） The level of protein expression for LI-cadherin in the SiRNA transfect group is significantly lower than the control group and the empty plasmid carrying group.Conclusions LI-cadherin is crucial and important for the adhension capability of HCC in its migration.SiRNA transfected LI-cadherin increases the metastasis in a nude mouse model inoculated with human hepatocellular carcinoma cell lines.

关 键 词：RNA 小分子干扰 肝肿瘤 实验性 肝肠粘连蛋白 

分 类 号：R735.7[医药卫生—肿瘤]