矮壮素对银杏叶片光合代谢与萜内酯生物合成的影响  

Effects of Chlorocholine Chloride on Photosynthesis Metabolism and Terpene Trilactones Biosynthesis in The Leaf of Ginkgo biloba

作　　者：许锋[1] 张威威[1] 孙楠楠[1] 李琳玲[2] 程水源[2] 王燕[3] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000 [3]黄冈市林业局,湖北黄冈438000

出　　处：《园艺学报》2011年第12期2253-2260,共8页Acta Horticulturae Sinica

基　　金：湖北省自然科学基金项目(2006ABA005);湖北省自然科学基金创新群体项目(2011CDA117);湖北省教育厅高校产学研合作项目(CXY2009B009);湖北省科技攻关重大项目(2004AA204B03);荆州市科技发展计划项目(20041PB06)

摘　　要：为探讨矮壮素(CCC)调控银杏叶萜内酯生物合成的机理,以3年生银杏实生苗为试材,研究了0、0.5、1.0和2.0 g.L-1CCC处理对银杏叶光合作用、光合色素、可溶性糖和萜内酯含量的影响,并采用实时定量PCR技术(qRT-PCR)检测了对银杏萜内酯合成途径中5个关键基因表达水平的影响。结果表明,0.5、1.0和2.0 g.L-1 CCC处理均可显著提高银杏叶光合作用速率,气孔导度,细胞间CO2浓度,蒸腾速率,以及叶绿素、类胡萝卜素和可溶性糖的含量。在1.0和2.0 g.L-1CCC处理下,银杏内酯A、银杏内酯B、白果内酯和总萜内酯含量显著高于对照。qRT-PCR分析结果显示CCC处理能显著上调银杏内酯合成途径中4个关键基因(DXS、DXR、GGPPS、LPS)的表达,表明CCC在分子水平上可能是通过诱导内酯合成关键基因表达来促进内酯的生物合成。In order to study the regulation mechanism of chlorocholine chloride（CCC）on the biosynthesis of terpene trilactones,effects of CCC on photosynthesis,the contents of photosynthetic pigment,soluble sugar and terpene trilactones,and the expression level of key genes involved in ginkgolide biosynthesis in Ginkgo biloba leaves were investigated.The three-year-old ginkgo seedlings were foliar sprayed with 0（control）,0.5,1.0 and 2.0 g.L-1 CCC.The results showed that 0.5,1.0,and 2.0 g.L-1 CCC treatments significantly enhanced the net photosynthetic rate,stomatal conductance,intercellular CO2 concentration,transpiration rate,and the contents of chlorophyll,carotenoids and soluble sugar in ginkgo leaves.The contents of ginkgolide A,B and bilobalide in ginkgo leaves treated with 1.0 and 2.0 g.L-1 CCC were significantly higher than those of control.The results of qRT-PCR analysis indicated that the expression levels of four key genes involved in terpene trilactones biosynthesis,namely DXS,DXR,GGPPS and LPS,were all markedly up-regulated by CCC treatment,indicating that CCC promoted terpene trilactones accumulation by inducing the expression of key genes related with ginkgolide biosynthesis.

关 键 词：银杏 矮壮素 光合作用 可溶性糖 萜内酯 关键基因 

分 类 号：S664.3[农业科学—果树学]