粪便Alu序列的检测在胰腺癌诊断中的价值  

Value of detection of fecal Alu repetitive sequences in the diagnosis of pancreatic cancer

作　　者：任艳[1] 高军[1] 王小玮[1] 刘建强[1] 顾俊骏[1] 金晶[1] 龚燕芳[1] 李兆申[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433

出　　处：《中华胰腺病杂志》2011年第6期383-385,共3页Chinese Journal of Pancreatology

基　　金：基金资助：国家科技支撑计划（2006BA102A12）

摘　　要：目的检测胰腺癌患者粪便Alu序列表达量，探讨其对胰腺癌的诊断价值。方法收集41例胰腺癌、27例慢性胰腺炎及23例健康者的粪便样本，采用酚一氯仿方法抽提粪便中基因组DNA，应用实时定量PCR方法检测Alu重复序列的表达量。结果胰腺癌、慢性胰腺炎、正常健康者粪便Alu重复序列表达量分别为（5．17±0．99）、（3．79±0．94）、（0．28±0．35）ng／g，三组间差异有统计学意义（P值均〈0．05）。通过接受者操作特征（ROC）曲线分析，胰腺癌的曲线下面积为74．8％，95％可信度为0．661～0．835，诊断胰腺癌的敏感性为75．6％，特异性为67．1％。结论胰腺癌患者粪便Alu序列表达量显著增加，对胰腺癌的诊断可能有一定价值。Objective To detect the Alu expression in the stool of patients with pancreatic cancer and investigate its value in the diagnosis of pancreatic cancer. Methods Stool samples were obtained from patients with pancreatic cancer （PC） （ n = 41 ）, chronic pancreatitis （CP） （ n = 27 ） and healthy subjects （ n = 23 ）, the DNA was extracted from the stool and the expression of Alu repetitive sequences was subjected to quantitative analysis by the real-time PCR. Results The expressions of Alu repetitive sequences in PC, CP, and healthy subjects were （5.17 ± 0.99）, （3.79 ±0.94）, （0.28 ±0.35 ） ng/g, and the difference among the three groups was statistically significant （P 〈0.05）. The AUC of PC was 74.8% with the 95% CI 0. 661 0. 835, and the sensitivity, specificity was 75.6% and 67.1%, respectively. Conclusions Alu repetitive sequences are highly expressed in the stool of patients with pancreatic cancer, and it is of value in the diagnosis of pancreatic cancer.

关 键 词：胰腺肿瘤 Alu序列 诊断 实时定量PCR 粪便 

分 类 号：R735.9[医药卫生—肿瘤]