胰腺癌内皮分化基因受体表达  

Expression of endothelial differemtiation gene receptors in human pancreatic cancer

作　　者：龚涌灵[1] 王卫东 王少开[1] 陶晨洁[1] 杨劲松[1] 田韧[1] 陈锦飞[1] 王书奎[1] 吕广梅 

机构地区：[1]南京医科大学附属南京第一医院肿瘤科、中心实验室,南京210006 [2]深圳第二人民医院急诊科 [3]江苏建康技术学院医护系

出　　处：《中华胰腺病杂志》2011年第6期396-399,共4页Chinese Journal of Pancreatology

基　　金：基金项目：南京市医学重点科技发展项目（ZKX09007）;江苏省卫生国际交流支撑计划;江苏省卫生厅科教兴卫工程医学领军人才与创新团队专项基金（苏卫科教2011字第15号）.

摘　　要：目的观察胰腺癌组织溶血磷脂酸（LPA）内皮分化基因受体（Edg／LPA）的表达，探讨其临床意义。方法收集50例胰腺癌及对应癌旁正常胰腺组织标本，采用实时定量PCR、免疫组化和蛋白质印迹法检测3种Edg／LPA受体亚型Edg-2／LPA1、Edg-4／LPA2和Edg-7／LPA3受体mRNA及蛋白的表达，分析其与胰腺癌临床病理参数的关系。结果胰腺癌组织Edg-2／LPA1、Edg-4／LPA2、EdgG／LPA3受体mRNA表达量分别为（0．142±0．042）％、（0．471±0．064）％、（0．231±0．043）％，对应癌旁正常胰腺组织分别为（0．132±0．029）％、（0．027±0．015）％、（0．163±0．046）％，其中胰腺癌组织的Edg-4／LPA2受体mRNA表达较对应癌旁组织显著增加（P〈0．05）。同样，胰腺癌组织Edg-4／LPA2蛋白表达也显著高于癌旁正常胰腺组织。胰腺癌组织的3种Edg／LPA受体mRNA表达水平与血清CA19-9浓度呈平行关系，差异无统计学意义。胰腺癌组织Edg-4／LPA2受体mRNA表达与肿瘤大小、导管腺癌的分化程度及侵袭转移有关，而Edg-2／LPA1、Edg-7／LPA3受体mRNA表达仅与肿瘤的侵袭转移有关。结论胰腺癌组织高表达Edg-4／LPA2受体，其高表达反映出肿瘤的恶性生物学行为。Objective To evaluate the expression of endothelial differentiation gene/lysophosphatidic acid （LPA） receptors （Edg/LPA） and its clinical significance in human pancreatic cancer. Methods Fifty cases of pancreatic cancer and adjacent normal tissues were collected, and Real-time PCR, Western blot and immunohistoehemistry was used to determine the expression of Edg-2/LPA1, Edg-4/LPA2 and Edg-7/LPA3 receptors mRNA and protein, and its relationship with clinieopathological parameters was analyzed. Results The expressions of Edg-2/LPA1, Edg-4/LPA2, Edg-7/LPA3 receptor mRNA were （ 0. 142 ± 0. 042 ） % , （0.471± 0. 064 ） %, （ 0.231±0. 043 ） % in pancreatic cancer, and the corresponding values were （ 0. 132 ± 0.029）% , （0.027 ±0.015）% , （0. 163 ±0.046）% in adjacent normal tissues. The expressions of Edg-4/ LPA2 receptor mRNA in pancreatic cancer were significantly lower than that in adjacent normal tissues （ P 〈 0.05 ）. The expressions of Edg-4/LPA2 receptor protein in pancreatic cancer were significantly lower than that in adjacent normal tissues （ P 〈 0.05 ）. The expressions of three types of Edg /LPA receptor mRNA in pancreatic cancer were parallel to serum CA19-9 levels. The expressions of Edg-4/LPA2 receptor mRNA were associated with tumor size, differentiation degree, and invasive ability and metastasis. While the expressions of Edg-2/LPA1, Edg-7/LPA3 receptor mRNA was associated with invasive ability and metastasis only. Conclusions Edg-4/LPA2 receptor is highly expressed in pancreatic cancer, which suggesting the malignantbiological behavior of pancreatic cancer.

关 键 词：胰腺肿瘤 溶血磷脂酸 Edg/LPA受体 

分 类 号：R735.9[医药卫生—肿瘤]