HLA-G在结肠癌外周血中的表达及其临床意义  

Expression of HLA-G mRNA on peripheral blood mononuclear cell in colon cancer patients and its clinical significance

作　　者：徐亦君[1] 竺明晨[2] 

机构地区：[1]南京医科大学附属南京第一医院消化科,江苏南京210006 [2]江苏省肿瘤医院检验科

出　　处：《中国肿瘤外科杂志》2011年第6期348-350,共3页Chinese Journal of Surgical Oncology

基　　金：江苏省肿瘤医院青年创新研究项目(ZQ200904)

摘　　要：目的检测组织相容性白细胞抗原G(HLA-G)在结肠癌外周血单个核细胞(PBMC)中的表达并探讨其临床应用价值。方法采用实时荧光定量PCR相对定量检测结肠癌患者、结肠良性病变患者和健康对照外周血单个核细胞HLA-G mRNA的表达。结果外周血单个核细胞HLA-G mRNA在结肠癌、结肠良性病变和健康对照中的相对表达量95%可信区间(CI)分别为[1.15,2.68]、[0.56,1.51]和[0.66,1.41]。结肠癌患者外周血单个核细胞HLA-G mRNA的表达高于结肠良性病变(P=0.02)和健康对照(P=0.03)。结论结肠癌患者外周血单个核细胞HLA-G与结肠癌发生密切相关,在结肠癌早期诊断中具有重要作用。Objective To evaluate the expression of HLA-G mRNA in colon cancer and its significance. Methods The expression of HLA-G mRNA on PBMC was determined by real-time PCR. Results The relative expression ratio CI of PBMC HLA-G mRNA was [ 1.15, 2.68] in colon cancer, [0.56, 1. 51 ] in colon benign lesion, [0.66, 1.41 ] in healthy individuals, respectively. The expression level of HLA-G mRNA on PBMC in colon cancer was significantly higher than that in both benign colon lesion （P = 0.02）and healthy con- trol （P = 0.03）. Conclusions HLA-G mRNA on PBMC in colon cancer was closely correlated with tumorigenesis and might play a crucial role in the colon cancer early diagnosis.

关 键 词：HLA-G 外周血单个核细胞 结肠癌 PCR 结肠良性病变 

分 类 号：R735.35[医药卫生—肿瘤]