色素上皮衍生因子抑制脉络膜新生血管的实验研究  

Experimental Study on the Inhibition of PEDF on CNV

作　　者：张喜梅[1] 李栋[1] 

机构地区：[1]山西省眼科医院,山西太原030002

出　　处：《山西职工医学院学报》2011年第4期1-3,共3页Journal of Shanxi Medical College for Continuing Education

基　　金：山西省科技厅青年科技基金项目资助(编号20041046)

摘　　要：目的:观察重组人色素上皮衍生因子(recombinant human Pigment Epithelium-Derived Factor,rhPEDF)对氩激光诱导的棕色挪威(brown Norway,BN)大鼠脉络膜新生血管的抑制作用。方法:对2组10只BN大鼠单眼行氩激光视网膜光凝,建立脉络膜新生血管模型。光凝后1周,一组动物玻璃体内注射2μg rhPEDF/10μLPBS;另一组动物玻璃体内注射10μL的PBS作为对照组。光凝后2周,对2组动物进行眼底荧光血管造影(fun-dus fluorescence vascular imaging,FFA)、组织病理学检查及光镜下测量脉络膜新生血管(choroidal neovasculariza-tion,CNV)厚度并对FFA结果及CNV厚度进行图像及统计学分析,以评估rhPEDF对脉络膜新生血管的抑制效果。结果:视网膜光凝后2周,FFA检查对照组与rhPEDF组相比,荧光素渗漏明显增加,差异有统计学意义(P<0.05);CNV厚度检测结果,rhPEDF组光凝区CNV厚度低于对照组,差异有统计学意义(P<0.05)。结论:对氩激光诱导形成CNV的BN大鼠玻璃体腔内注射rhPEDF,可以抑制CNV的增殖。Objective： To observe the inhibition of rhPEDF on CNV of BN rats induced by argon laser. Methods： 10 BN rats divided into two groups were photocoagulated by argon laser in single eye to set up the CNV model. 1 week later, one group of 5 rats were received intravitreal injections of 2ug rhPEDF/10ul PBS, and the other group of 5 rats were received intravitreal injections of 10ul PBS as control group. 2 weeks later, FFA and histopathologic examination were performed in two groups and the thickness of laser-induced CNV was measured by light microscope. The results of the FFA and CNV thickness were statistically analyzed to assess the inhibitory effect of rhPEDF on CNV. Results：2 weeks later after photocoagulation, FFA showed less iluorescein leakage in the rhPEDF group than that in the control group. There was statistical difference between the two groups （P 〈 0. 05 ）. The thickness of CNV in rhPEDF group was less than that in the control group. There was statistical difference between the two groups （ P 〈 0.05 ）. Conclusion： Intravitreal rhPEDF injection can inhibit CNV induced by argon laser in BN rat.

关 键 词：脉络膜新生血管 人重组色素上皮衍生因子 抑制 

分 类 号：R774.1[医药卫生—眼科]