FGF-2对体外培养人牙髓细胞FGFR和OPN表达活性的影响  

Effect of FGF-2 on FGFR and OPN expression of human dental pulp cells in vitro

作　　者：李桥[1] 汪敏[1] 韦永珍 孙虹[1] 潘乙怀[3] 

机构地区：[1]温州医学院,浙江温州325027 [2]黔西南州人民医院口腔科,贵州兴义562400 [3]温州医学院附属口腔医院,浙江温州325027

出　　处：《牙体牙髓牙周病学杂志》2011年第12期675-678,共4页Chinese Journal of Conservative Dentistry

基　　金：浙江省教育厅科研基金资助项目(Y200907095);浙江省大学生科技创新活动计划(新苗人才计划)资助项目(2011R413040)

摘　　要：目的:研究成纤维细胞生长因子-2(fibroblast growth factor-2,FGF-2)对体外培养的人牙髓细胞表面成纤维细胞生长因子受体(fibroblast growth factor receptor,FGFR)及骨桥蛋白(osteopontin,OPN)表达的影响。方法:以改良组织块培养法体外获得人牙髓细胞,采用Western blotting法检测不同浓度FGF-2作用下牙髓细胞FGFR的表达情况;应用Real Time-PCR法,检测不同浓度FGF-2作用下牙髓细胞OPN mRNA的表达。结果:与对照组相比,FGF-2在1～50 ng/mL浓度下均可促进牙髓细胞FGFR的表达(P<0.05),最佳显效浓度为10 ng/mL。FGF-2在1～50 ng/mL浓度下均可诱导牙髓细胞OPN mRNA表达(P<0.05),OPN mRNA的表达在10 ng/mL的FGF-2作用下达到高峰。结论:FGF-2可促进体外培养人牙髓细胞FGFR和OPN的表达,在牙髓牙本质复合体修复中可能发挥重要作用。AIM： To investigate the effect of fibroblast growth factor-2（FGF-2） on fibroblast growth factor receptor（FGFR） and osteopontin（OPN） expression of human dental pulp cells in vitro.METHODS： Human dental pulp cells were isolated from dental pulp tissue explants.FGFR expression of human dental pulp cells were examined by Western blotting assay.Real Time-PCR method was used to observe the OPN gene expression of human dental pulp cells.RESULTS： FGFR expression of human dental pulp cells was increased when FGF-2 was used at the concentrations of 1-50 ng/mL in comparison with that of the control cells（P 0.05）.The effect of FGF-2 on the FGFR expression reached its maximum at the concentration of 10 ng/mL.FGF-2 at concentrations of 1-50 ng/mL markedly induced OPN gene expression of dental pulp cells（P 0.05）.CONCLUSION： FGF-2 has the capability of promoting FGFR and OPN expression of human dental pulp cells in vitro,and may play an important role in the repair of pulpodentinal complex.

关 键 词：成纤维细胞生长因子-2 人牙髓细胞 成纤维细胞生长因子受体 骨桥蛋白 

分 类 号：R780.2[医药卫生—口腔医学]