灵芝孢子提取物对Lactacystin致PC12细胞损伤的保护作用  

PROTECTIVE EFFECTS OF GANODERMA LUCIDUM SPORE EXTRACT ON LACTACYSTIN INDUCED PC12 CELLS DAMAGE

作　　者：张红[1] 王芳[1] 刘湧[1] 王建东[2] 

机构地区：[1]成都医学院,成都610083 [2]四川省农业科学院土壤肥料研究所

出　　处：《现代预防医学》2012年第1期116-118,共3页Modern Preventive Medicine

基　　金：四川省财政育种工程2009年度优秀论文基金项目(2009YXLWJJ-009);成都医学院基金CYZ09-015

摘　　要：[目的]探讨灵芝孢子提取物对Lactacystin诱导PC12细胞损伤的保护作用。[方法]体外培养PC12细胞,建立Lactacystin诱导PC12细胞损伤的模型,观察细胞形态,用CCK-8法检测细胞活力,annexin V-FITC/PI流式细胞法检测细胞调亡。[结果]用不同浓度的灵芝孢子提取物处理PC12细胞时,细胞存活率与对照几乎一致,并未表现出细胞毒性。经20μmol/L的Lactacystin处理24 h后,PC12细胞活力比对照组降低,仅为对照组的63.2%。模型组经不同浓度的灵芝孢子提取物预处理后,细胞活力明显提高,其保护作用随浓度升高而升高。Lactacystin诱导PC12细胞凋亡,处理24 h后细胞凋亡率为48.86%,经灵芝孢子提取物处理后,细胞凋亡率明显降低。[结论Lactacystin能诱PC12细胞凋亡,灵芝孢子提取物能对Lactacystin致PC12细胞损伤有一定的保护作用。[Objective]To investigate the effect of Ganoderma lucidum spore extract（GLS extract）on PC12 cells damage iduced by Lactacystin.[Methods]The toxicity model was established by treating PC12 cells with Lactacystin in vitro.Observed the morphological changes of cells by microscope.Cell viability was assayed with CCk-8 method.The cell apoptotic rate was measured by flow cytometry（FCM）method.[Results]After being treated with different concentrations of GLS extract,the viability of PC12 cells as same as the cell control group tested by CCK-8 assay.After being treated with Lactacystin（20？mol/L）for 24 h,the viability of PC12 cells depressed and the viable cells was only 63.2% when compared with cell control group.Pretreated with GLS extract at different concentrations,the cell viabilities were all increased significantly.The protective effect on cells increased with its concentration increased.Lactacystin induced apoptosis in PC12 cells,and the apoptotic rate was 48.86% after 24 h of treatment.Pretreated with GLS extract,the cell apoptotic rates reduced.[Conclusion] Lactacystin can induce apoptosis in PC12 cells,while GLS extract can protect PC12 cells from the injury induced by Lactacystin.

关 键 词：灵芝孢子提取物 LACTACYSTIN PC12细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]