重组血管内皮生长因子165对肝硬化大鼠肝功能的影响  

Effect of Recombinant Vascular Endothelial Growth Factor 165 on the Liver Function in Cirrhotic Rats

作　　者：于良立[1] 赵金满[1] 蒋伟伟[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,沈阳110001

出　　处：《中国医科大学学报》2011年第12期1092-1095,共4页Journal of China Medical University

摘　　要：目的探讨经门静脉导入重组血管内皮生长因子165对肝硬化大鼠肝功能的影响。方法雄性SD大鼠50只,体质量180~220 g,完全随机分成正常组10只、诱导模型组40只。采用硫代乙酰胺诱导肝硬化模型方法,10周后成模25只。模型组随机分为肝硬化实验组15只和肝硬化对照组10只。实验组经门静脉插管植入Alzet微渗泵灌注血管内皮生长因子入门静脉,持续作用2周。正常组和肝硬化对照组行开腹后关腹对照处理。2周后处死各组大鼠,HE染色观察大鼠肝脏病理组织学改变;全自动生化分析仪检测血清中的丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、白蛋白（ALB）和总胆红素（TBIL）含量。结果肝硬化对照组肝细胞变性坏死、弥漫纤维结缔组织增生,有假小叶形成。而肝硬化实验组变性坏死程度减轻,纤维结缔组织增生范围缩小,纤维化分级有明显改善,差异具有统计学意义（P〈0.01）。肝硬化对照组ALT、AST、TBIL均显著升高,ALB下降,同正常组相比差异具有统计学意义（P〈0.01）。肝硬化实验组同肝硬化对照组比较,ALT、AST、TBIL均显著下降,ALB上升,差异具有统计学意义[（115.71±12.63）U/L vs（192.36±21.84）U/L;（196.26±18.45）U/L vs（295.11±31.78）U/L;（6.32±1.32）μmol/L vs（10.69±2.47）μmol/L;（14.57±1.92）g/L vs（9.90±1.27）g/L;P〈0.01）]。结论经门静脉灌注血管内皮生长因子165具有改善肝硬化大鼠肝脏功能的作用。Objective To investigate the effect of portal vein infusion of recombinant vascular endothelial growth factor 165 on liver function in cirrhotic rats.Methods Fifty male SD rats,weighting 180-220 g,were randomly divided into normal group（n = 10） and model group（n = 40）.The model group was used to induce cirrhosis using the thioacetamide approach.After 10 weeks,25 cirrhotic rats were randomly divided into experimental group（n = 15） and model control group（n = 10）.The experimental group was intubated for implantation of an Alzet osmotic pump,which was used to infuse recombinant VEGF165 via the portal vein for 2 weeks.The normal group and model control group underwent sham operation.All rats were killed after 2 weeks,and the pathological changes in liver tissue were observed by HE staining.Serum levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,albumin（ALB） and total bilirubin（TBIL） content were measured by automatic biochemical analyzer.Results Degeneration and necrosis of liver cells,diffused proliferation of fibrous connective tissue and the formation of pseudo lobules appeared in the model control group.In the experimental group,degeneration and necrosis of liver cells were milder and the rate of liver fibrosis was improved significantly compared to the model control group（P 0.01）.Compared to the normal group,levels of ALT,AST,TBIL in the serum of cirrhosis control group were significantly increased,while ALB decreased;the difference was statistically significant（P 0.01）.Levels of ALT,AST,TBIL in the serum of cirrhosis experimental group were significantly decreased,while ALB increased;the difference was statistically significant（115.71±12.63 U/L vs 192.36±21.84 U/L;196.26±18.45 U/L vs 295.11±31.78 U/L;6.32±1.32 μmol/L vs 10.69±2.47 μmol/L;14.57±1.92g/L vs 9.90±1.27 g/L;P 0.01）.Conclusion Portal vein infusion of VEGF165 can improve the liver function in rats with cirrhosis.

关 键 词：肝硬化 血管内皮生长因子 肝功能 

分 类 号：R575.2[医药卫生—消化系统]