刚果红与β-环糊精及其衍生物的包合物与DNA的作用研究(英文)  

Interaction between Inclusion Complexes of β-Cyclodextrins/Congo Red and DNA by UV-visible and Fluorescence Spectra

作　　者：唐斯萍[1,2] 曾朵清[2] 黄耿[1,2] 冯泳兰[1,2] 许志锋[1,2] 庾江喜[1,2] 

机构地区：[1]湖南省普通高等学校功能金属有机材料重点实验室,湖南衡阳421008 [2]衡阳师范学院化学与材料科学系,湖南衡阳421008

出　　处：《衡阳师范学院学报》2011年第6期74-78,共5页Journal of Hengyang Normal University

基　　金：Supported by the National Natural Science Foundation of China(21102040);The Scientific Research Fund of Hunan Provincial Education Department(09K099);The Foundation of science and technology of Hengyang(2010kj26);Science Foundation of Hengyang Normal University of China(10B66);Foundation for University Key Teacher by Hunan Provinc

摘　　要：用荧光光谱法、紫外-可见分光光度法在生理条件下(pH=7.41)研究了β-环糊精(β-CD)、6-去氧-(N-胺乙基)环糊精(CR-β-CDen)分别与刚果红(CR)的包合作用;研究了CR,(CR-β-CD)和(CR-β-CDen)超分子体系与小牛胸腺DNA的相互作用。紫外滴定实验结果表明:(1)β-环糊精和6-去氧-(N-胺乙基)环糊精都与客体分子刚果红发生超分子作用;(2)CR,CR-β-CD,CR-β-CDen与小牛胸腺DNA作用的结合常数Kb值分别为4.1×104L/mol,1.36×105L/mol,2.33×105L/mol,表明超分子体系CR-β-CDen与DNA的相互作用最强。荧光实验结果表明三种化合物CR,CR-β-CD,CR-β-CDen的荧光强度随着CT-DNA的增加而增大,其中环糊精衍生物CR-β-CDen荧光增强效果是最好的。Inclusion interaction of β-cyclodextrin （β-CD） and 6-mono- （N-aminoethyl） amino-β-cyclodextrin （9-CDen） with congo red and interaction of congo red （CR） and inclusion complexes β-CD/CR and β-CDen/CR with calf thymus DNA are researched by UV-vis and fluorescence spectra. The results show that the values of the binding constants （Kb） of CR/ DNA, CR-β-CD/DNA and CR-β-CDen/DNA are 4.1 × 10^4 L/tool, 1.36 × 10^5 L/mol and 2.33× 10^5 L/mol respectivly, which indicates that the interaction between CR-β-CDen and DNA is the strongest. The three complexes luminescence were enhanced with addition of CT-DNA. And the effect of fluorescence enhancement of CR-β-CDen is the best.

关 键 词：Β-环糊精 刚果红 包合物 小牛胸腺DNA 

分 类 号：O636.12[理学—高分子化学]