碱性亚硫酸盐预处理对桉木酶解糖化的影响  

Effect of the Alkaline Sulfite Pretreatment on the Enzymatic Saccharification of Eucalyptus Wood

作　　者：尉慰奇[1] 武书彬[1] 刘立国 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]广州优锐生物科技有限公司,广东广州510640

出　　处：《林产化学与工业》2011年第6期29-34,共6页Chemistry and Industry of Forest Products

基　　金：广州市科技计划项目资助(2010y1-C071)

摘　　要：为提高按木原料的酶解糖化效果,采用碱性亚硫酸盐对桉木原料进行预处理,并对预处理所得样品进行纤维素酶酶解糖化。通过测定酶解液中的葡萄糖、木糖得率,探讨预处理条件对产糖得率的影响。结果表明:随着蒸煮温度的升高,NaOH用量的增大,桉木酶解液中木糖、葡萄糖得率明显增大;随着Na2SO3用量的增大,保温时间的延长,酶解液中木糖、葡萄糖得率先增大后减小。最佳预处理条件为:蒸煮最高温度170℃,保温时间3 h,NaOH用量3%,Na2SO3用量10%。此时,酶解木糖、葡萄糖得率分别可达15.37%和60.79%,比未经处理的1.73%和12.33%提高了7.88倍和3.93倍。In order to improve the effect of enzymatic saccharification of eucalyptus wood, eucalyptus lignocellulose was pretreated by an alkaline sulfite pretreatment. The obtained samples were conducted for cellulose enzymolytic saccharification. According to the sugars yield in the enzymolysis liquid, the influence of pretreatment conditions on the reducing sugars yield was discussed. The results showed that with the increases of cooking temperature and NaOH dosage, both the glucose and xylose yields increased obviously in the enzymolysis liquid. Both glucose and xylose yields increased at first, then decreased. When the heating time and Na2SO3 dosage increased. The optimal pretreatment conditions are as follows： cooking temperature 170 ℃, sodium sulfite charge 10 %, sodium hydroxide charge 3 %, reaction time of temperature keeping 3 h. In addition, the yields of glucose and the xylose reached 60.79 % and 15.37 %, which are 3.93 and 7.88 times higher than that of the untreated, respectively.

关 键 词：桉木 碱性亚硫酸盐预处理 酶解 糖得率 

分 类 号：TQ351[化学工程]