松树皮多酚的亚临界水提取及抗氧化活性初探  

Preliminary Study on Subcritical Water Extraction and Antioxidant Activity of Polyphenols from Pine Bark

作　　者：薄采颖[1,2,3] 郑光耀[1,2,3] 陈琰[4] 周维纯[1,2,3] 李若达 谢学容 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]中国药科大学药理学教研室,江苏南京210009 [5]中健行集团有限公司,广东乐昌512229

出　　处：《林产化学与工业》2011年第6期73-77,共5页Chemistry and Industry of Forest Products

基　　金：国家林业局948技术引进项目(2008-4-80);江苏省产学研前瞻性联合研究项目(BY2011116)

摘　　要：采用亚临界水从松树皮中提取多酚,考察了反应条件对多酚得率的影响,并与超声波辅助提取法和热回流提取法进行了比较,同时测定了亚临界水提取所得松树皮提取物的DPPH自由基清除能力。结果表明:亚临界水提取松树皮多酚的最佳工艺条件为:提取温度150℃、提取时间5 min、液料比20∶1(mL∶g)、提取压力4 MPa,多酚的提取得率为4.86%,1次提取且相同液料比条件下,比超声波辅助提取法和热回流提取法分别提高了33.52%和37.68%。亚临界水提取所得松树皮提取物对DPPH自由基具有良好的清除效果,其半数抑制质量浓度(IC50)为7.10 mg/L,抗氧化性高于常用抗氧化剂BHA(7.82 mg/L)。Subcritical water was used to extract polyphenol from pine bark. Its effects on the polyphenol yield were investigated. The DPPH radical scavenging activity of polyphenol by subcritical water extraction was determined. The results showed that the optimum process conditions were extraction temperature 150 ℃, extraction time 5 min, liquid-feed ratio 20： 1, and extraction pressure 4 MPa. Under this condition the yield of polyphenol was 4.86 %. Compared with the ultrasound-assisted extraction and heat reflux extraction, the subcritical water extraction improved the yields of polyphenol by 33.52 % and 37.68 % , respectively. The polyphenol extracted by subcritieal water had a better DPPH radicals scavenging activity than that of the antioxidant BHA （7.82 mg/L）. The half inhibitory concentration （IC50） of the polyphenol was 7.10 mg/L.

关 键 词：松树皮 亚临界水 多酚 DPPH 

分 类 号：TQ351.0[化学工程]