膜相关蛋白Numb在结肠癌中的表达和意义  

Numb Expression in Colon Cancer and Its Significance

作　　者：曾蕴林[1,2] 邵喜明[1,2] 李华顺[1,2] 

机构地区：[1]四川大学华西第二医院发育干细胞研究所,成都610041 [2]四川大学华西医院生物治疗国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2012年第1期6-8,14,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30771102)资助

摘　　要：目的探讨膜相关蛋白Numb在结肠癌组织中的表达和生物学意义。方法对63例结肠癌和20例正常结肠组织的组合组织芯片进行常规免疫组化染色,检测Numb蛋白的表达,结合临床病理资料进行分析。采用免疫荧光染色和Westen blot检测结肠癌SW480、SW620细胞系中Numb的表达。结果低分化的结肠癌组织较正常结肠组织和中、高分化的结肠癌组织胞浆中Numb的表达阳性率下降(P<0.05),Numb表达强度与结肠癌分化程度相关。Westen blot结果显示,结肠癌细胞系中Numb的表达强度,SW480较SW620细胞系高(P<0.05)。激光共聚焦免疫荧光染色观察,Numb呈点状分布于胞质和包膜,SW480较SW620细胞系在胞浆中分布更密集。结论 Numb可能在肿瘤进展过程中起保护作用,对肿瘤的发生发展有重要调控。Objective To investigate the expression pattern of Numb,a membrane associated protein,in colon cancer and its biological significance.Methods Routine immunohistochemistry staining was used to detect the expression of Numb in tissue microarray with 60 cases of colon cancer tissue and 20 normal colon tissue.The clinical data was analyzed.The expression of Numb in SW480,SW620 cell lines were evaluated by immunostaining and Western blot.Results The positive expression rate of Numb in cytoplasm decreased in poorly differentiated tumor tissue than normal tissue and well and moderately differentiated colon cancer（P〈0.05）,and the expression intensity was correlated with the differentiation state of cancer tissue.Westen blot results showed that colon cancer cell line SW480,compared with SW620 has a higher protein expression（P〈0.05）.A punctate distribution of Numb in cytoplasm in colon cancer cell under confocal immunofluorescence was observed.A increased expression density in SW480 cell line was noticed when compared with that in SW620 cell line（P〈0.05）.Conclusion Numb may has an important regulation in the process of tumor progression and play a protective role against tumor development.

关 键 词：NUMB 结肠癌 SW480 SW620 

分 类 号：R735.35[医药卫生—肿瘤]