Pg-LPS对兔单核/巨噬细胞炎症因子表达的影响  

Effects of Porphyromonas gingivalis-lipopolysacchride on inflammatory factors in monocytes/macrophages from rabbits

作　　者：游晓庆[1] 李厚轩[1] 吴春芳[1] 许雯静[1] 李艳芬[1] 闫福华[1] 

机构地区：[1]福建医科大学口腔医学院牙周科福建省高校口腔医学重点实验室,福州350002

出　　处：《免疫学杂志》2012年第1期10-14,共5页Immunological Journal

基　　金：国家自然科学基金(30973326);福建省自然科学基金青年创新课题(2009J05064);福建医科大学临床医学重点学科建设项目(XK201111)

摘　　要：目的比较Pg-LPS对新西兰兔不同部位单核/巨噬细胞炎症因子(IL-1β、IL-6、TNF-α)表达的影响。方法分离新西兰兔不同部位(血液、肺、腹腔、肝脏)的单核/巨噬细胞(Mo、AM、PM、KC),将每一个部位的细胞分别用E.coli-LPS、Pg-LPS刺激。运用RT-PCR法检测各组Mo、AM、PM、KC中IL-1β、IL-6、TNF-αmRNA的表达情况。结果各实验组的4个部位(Mo、AM、PM、KC)相比,IL-1β、IL-6、TNF-αmRNA的表达均存在部位差异(P﹤0.05)。E.coli-LPS组和Pg-LPS组IL-1β、IL-6、TNF-αmRNA的表达较对照组总体上均明显升高(P﹤0.05),并且E.coli-LPS组的升高作用强于Pg-LPS组(P﹤0.05)。结论不同部位的单核/巨噬细胞对Pg-LPS的刺激存在敏感性差异。Pg-LPS可以增强单核/巨噬细胞炎症因子基因的表达水平。In order to compare the effect of Porphyromonas gingivalis lipopolysacchride on the inflammatory factors（interlenkin-1β,interlenkin-6,and tumor necrosis factor-α） in monocytes/macrophages of different parts from the New Zealand rabbits,we isolated peripheral mononuclear cells,alveolar macrophages,peritoneal macrophages and kupffer cells.Then the cells from each part were stimulated with E.coli-LPS and Pg-LPS,subsequently the mRNA expressions of IL-1β,IL-6 and TNF-α were analyzed through RT-PCR.The monocytes/macrophages of different parts showed distinct response of inflammatory factors in all groups（P﹤0.05）.We also found that compared with the control,E.coli-LPS and Pg-LPS induced higher mRNA expression of inflammatory factors（IL-1β,IL-6,TNF-α,P﹤0.05）,but the intensity of E.coli-LPS group was more significant than Pg-LPS group（P﹤0.05）.In conclusion,Pg-LPS-activated monocytes/macrophages show site-specific reaction.Periodontal pathogen Pg-LPS could increase the gene levels of inflammatory factors in monocytes/macrophages.

关 键 词：牙周病 脂多糖 单核/巨噬细胞 炎症因子 

分 类 号：R781.4[医药卫生—口腔医学]