5-羟色胺转运体敲除小鼠在应激下焦虑行为和HPA轴的改变  

Change of anxious behavior and hypothalamic-pituitary-adrenal axis in serotonin transporter knockout mice under stress

作　　者：蒋雪[1] 邓永兵[2] 安娜[1] 吴利亚[1] 

机构地区：[1]重庆医科大学基础医学院生物化学与分子生物学教研室,重庆400016 [2]重庆市急救医疗中心神经外科,重庆400014

出　　处：《第三军医大学学报》2012年第1期58-61,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(81001017);重庆市教育委员会科技基金(教科文2010-5)~~

摘　　要：目的研究5-羟色胺转运体(serotonin transporter,SERT)敲除小鼠焦虑行为及ACTH和皮质酮分泌变化。方法将基因型为SERT+/-的雌雄小鼠交配产子,并把鉴定后子代小鼠按基因型(SERT+/+、SERT+/-、SERT-/-)分为对照组和EPM组,每组15只。利用高架十字迷宫作为应激原刺激小鼠,观察各组小鼠的焦虑行为,并测定各组小鼠的ACTH和皮质酮分泌。结果①基因型PCR成功鉴定SERT+/+、SERT+/-和SERT-/-的小鼠。②SERT+/-和SERT-/-小鼠的开臂停留时间、开臂停留时间百分比、进入开臂的次数和进入开臂的次数百分比较SERT+/+均显著降低(P<0.05,P<0.01)。③EPM刺激下,SERT+/-和SERT-/-小鼠ACTH分泌的增加显著高于SERT+/+(P<0.05),SERT-/-小鼠皮质酮分泌的增加显著高于SERT+/+(P<0.05)。结论 SERT敲除小鼠在应激条件下易产生焦虑,并且ACTH和皮质酮分泌显著升高。Objective To study the change of anxious behavior and secretion of adrenocorticotropic hormore （ACTH） and corticosteron in serotonin transporter （SERT） knockout mice. Methods Heterozygous SERT mutant mice （SERT ＋/- ） were mated to produce offspring. Thirty offspring mice were divided into control group （n = 15 ） and elevated plus maze （EPM） group （ n = 15 ） according their genotype （ SERT＋/＋ , SERT＋/-, SERT-/- ）. The mice were stimulated with EPM as a stressor and their anxious behaviors were observed. ACTH and corticosteron levels in the mice were measured. Results （ 1 ） The genotypes were iden- tiffed in SERT＋/＋ , SERT＋/ and SERT-/- mice respectively by PCR. （2） The open arm time （OT） , the OT %, the open arm entry （OE） and OE% were significantly lower （P 〈0.05, P 〈0.01 ）. （3） The ACTH level was significantly higher in SERT＋/- and SERT-/- mice than in SERT＋/＋ mice after EPM stimulation （ P 〈 0.05 ） while the eorticosteron level was significantly higher in SERT -/- mice than in SERT ＋/＋ mice （ P 〈 0.05）. Conclusion SERT knockout mice are easy to have anxiety with their ACTH and corticosteron level significantly increased uuder stress.

关 键 词：5-羟色胺转运体 高架十字迷宫 ACTH 皮质酮 敲除小鼠 

分 类 号：R749.720.2[医药卫生—神经病学与精神病学] R394-33[医药卫生—临床医学]