大鼠溃疡性结肠炎模型结肠组织的代谢组学研究  

Study of metabonomics on the tissue in ulcerative colitis rat model

作　　者：雅森.米吉提 阿地里江.阿布力米提 库热西.玉努斯 巴图尔.买买提明 

机构地区：[1]新疆医科大学,乌鲁木齐830011 [2]新疆医科大学基础医学院生物化学与分子生物学教研室,乌鲁木齐830011 [3]新疆医科大学药学院分析测试中心,乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第12期1350-1354,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金资助项目(编号:30460163)

摘　　要：目的应用代谢组学研究技术测试与比较正常对照组和大鼠溃疡性结肠炎模型(Ulcerative ColitisModel,UCM)组结肠组织代谢产物,探讨UCM结肠组织中UC相关的特异性分子标志物。方法将Wistar大鼠随机分为正常对照组(22只)和溃疡性结肠炎模型组(21只),分笼喂养,采用2,4-二硝基氯苯(2,4-dinitrochloro-benzene,DNCB)复合乙酸法复制大鼠溃疡性结肠炎动物模型,收集两组大鼠结肠组织,进行1H-NMR图谱测定和主成分分析。结果与正常对照组大鼠相比,UC模型组结肠组织的内源性代谢谱、肠组织的代谢表型发生了显著变化,UC模型组大鼠结肠组织中丙酸、乳酸、牛磺酸、乙酸、脯氨酸、丙氨酸等小分子代谢物含量明显增加,差异有统计学意义(P<0.05)。结论 UC大鼠结肠组织的代谢谱明显偏离正常大鼠结肠组织,乳酸、牛磺酸、脯氨酸、丙氨酸等6种代谢物在临床上可作为溃疡性结肠炎早期诊疗的分子标志物。Objective To explore the molecular marker in the UC through application of Metabolomics technology testing and to compare between the normal control group and the Ulcerative Colitis Model group on rat. Methods The animals are divided into two groups at random, which are normal control group （n=21） and model group （n=22）, firstly establish the ulcerative colitis rat model by “DNCB＋Acetic acid” , the plasma of two group’s are collected and its 1H nuclear magnetic resonance （1H-NMR） spectra are acquired. Results Compared with normal group and model group’s Propionic Acid, lactic acid, alanine, acetic acid, praline, taurine and Unknown substance are increased. Conclusion The metabolic spectrum in the rats with Ulcerative Colitis is found greatly different from those in the normal rats. What’s more, lactic acid, alanine, acetic acid, praline, and taurine may serve as peculiar molecular markers in the UC colon tissue.

关 键 词：溃疡性结肠炎 代谢组学 分子标志物 

分 类 号：R574.62[医药卫生—消化系统]