海参虫草复合物降血糖及机制研究  

THE HYPOGLYCEMIC EFFECT OF MIXTURE OF APOSTICHOPUS JAPONICUS AND CORDYCEPS MILITARIS AND ITS MECHANISM

作　　者：柳东[1] 王静凤[1] 薛勇[1] 王玉明[1] 傅佳[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《营养学报》2011年第6期589-593,596,共6页Acta Nutrimenta Sinica

基　　金：国家自然基金资助项目(No.30972284);海洋公益性行业科研专项(No.201105029);国际科技合作项目(No.2010DFA31330);"泰山学者建设工程专项经费"

摘　　要：目的探讨海参虫草复合物(Apostichopus japonicus and Cardyceps millitaris,AC)对糖尿病大鼠的降血糖作用。方法通过一次性腹腔注射链脲佐菌素(streptozocin,STZ)建立糖尿病大鼠模型,将成模大鼠随机分为模型对照组、海参虫草复合物低剂量组、高剂量组。灌胃海参虫草复合物35 d后,分别检测大鼠空腹血糖值和血清胰岛素含量;采用RT-PCR法测定肌肉及脂肪组织中磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)、蛋白激酶(serine-threonine kinases,Akt)和葡萄糖转运蛋白4(glucose transporter 4,Glut4)mRNA的表达,Western blot方法检测肌肉及脂肪中Glut4蛋白的表达。结果海参虫草复合物能显著降低糖尿病大鼠空腹血糖(P<0.01),增加血清胰岛素含量(P<0.01),上调肌肉及脂肪组织中PI3K、Akt和Glut4的mRNA表达(P<0.05或P<0.01),增高脂肪组织中Glut4的蛋白表达水平(P<0.01)。结论海参虫草复合物具有降低血糖的作用,其机制可能与部分上调胰岛素介导的PI3K/Akt/Glut4信号转导通路有关。Objective To study the hypoglycemic activity of the mixture of Apostichopus japonicus and Cordyceps militaris （AC） in diabetic rats. Method Rat diabetic model was induced by a single intraperitoneal injection of streptozotocin Diabetic rats were randomly divided into diabetic control group, low-dose AC group and high-dose AC group. The diabetic rats were given different dosages of AC daily for 5 w. Each of the following indicators was inspected： the change of the level of fasting blood glucose and serum insulin contents. The mRNA expression levels of phosphatidylinositol 3-kinase （PI3K）, serine-threonine kinases （Akt） and glucose transporter 4 （Glut4） in skeletal muscle and adipose tissues were assessed by semi-quantity RT-PCR. The expression of Glut4 protein in skeletal muscle and adipose tissues were detected by Western blot analysis. Results The AC had remarkably suppressed hyperglycemia （P〈0.01）, and improved serum insulin contents （P〈 0.01）. The expressions of PI3K, Akt and Glut4 mRNA were markedly upregulated in skeletal muscle and adipose tissues, which were effectively suppressed by AC treatment （P〈0.05 or P〈0.01）. Western blot showed that the expression of Glut4 protein was improved in adipose tissues by AC treatment （P〈0.01）. Conclusion AC has hypoglycemic effect on diabetic rat through improvement of insulin signaling pathway of PI3K/Akt/Glut4.

关 键 词：日本刺参 蛹虫草 磷脂酰肌醇3-激酶 蛋白激酶Akt 葡萄糖转运蛋白4 

分 类 号：R285.5[医药卫生—中药学]