老年大鼠脑出血后海马齿状回神经干细胞的增殖与分化  

Proliferation and differentiation of neural stem cells in hippocampus dentate gyrus in aged rats after intracerebral hemorrhage

作　　者：文玉军[1] 王登科[2] 孙征[1] 刘海洋[1] 张莲香[1] 王效军[1] 秦毅[1] 

机构地区：[1]宁夏医科大学基础医学院人体解剖与组织胚胎学系,宁夏银川750004 [2]宁夏师范学院,宁夏固原756000

出　　处：《中风与神经疾病杂志》2011年第12期1070-1073,共4页Journal of Apoplexy and Nervous Diseases

基　　金：宁夏回族自治区自然科学基金资助项目(NZ0650);宁夏回族自治区教育厅重大科研项目(NJ20090093);宁夏医科大学面上项目;宁夏医科大学重点孵育项目(XZ201001)

摘　　要：目的观察老年大鼠脑出血后海马齿状回神经干细胞(NSCs)的增殖与分化,探讨脑出血后NSCs的变化规律。方法制作老年大鼠脑出血模型,5-溴脱氧尿核苷(BrdU)腹腔注射标记增殖细胞,用免疫组化法检测大鼠海马齿状回BrdU、神经元核抗原(NeuN)、胶质纤维酸性蛋白(GFAP)阳性细胞数的变化。结果正常组和假手术组老年大鼠海马齿状回均有少量BrdU阳性细胞,脑出血后大鼠各时间段的BrdU阳性细胞数目均较正常组和假手术组明显增加,7d组达到峰值后逐渐下降,28d组仍高于正常组和假手术组。正常老年大鼠海马齿状回可见少量BrdU/NeuN和BrdU/GFAP双标阳性细胞,脑出血后双标阳性细胞数较正常组明显增加。结论脑出血后老年大鼠海马齿状回NSCs增殖明显,且可以向神经元和神经胶质细胞分化。Objective To investigate proliferation and differentiation of neural stem cells（NSCs） in the hippocampus dentate gyrus in aged rats after intracerebral hemorrhage（ICH）,and to explore the variation of NSCs after ICH.Methods ICH models were established by injecting collagenase Ⅶ into the brain of aged rats.Proliferating cells were labeled by BrdU through abdominal cavity injection.Immunohistochemical single and double staining with antibodies agaist BrdU,NeuN and GFAP were used to determine proliferation and differentiation of hippocampus dentate gyrus.Results In non-ICH aged rats,BrdU positive cells in hippocampus dentate gyrus were little.After ICH,BrdU positive cells in hippocampus dentate gyrus of aged rats were significantly increased and reached peak after 7d.In non-ICH aged rats,BrdU/NeuN and BrdU/GFAP double positive cells were found in the hippocampus dentate gyrus.After ICH,the quantity of double positive cells increased obviously.Conclusion After ICH,NSCs in hippocampus dentate gyrus of aged rats are activated,which can proliferate and differentiate to neuron or astrocytes.

关 键 词：脑出血 海马齿状回 神经干细胞 增殖 分化 老年 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]