eNOS与蛛网膜下腔出血后脑血管痉挛的关系  

The relationship between eNOS and cerebral vasospasm after subarachnoid hemorrhage

作　　者：王灯亮[1] 康德智[1] 张元隆[1] 林章雅[1] 林元相[1] 余良宏[1] 

机构地区：[1]福建医科大学附属第一医院神经外科,福建福州350004

出　　处：《中风与神经疾病杂志》2011年第12期1094-1097,共4页Journal of Apoplexy and Nervous Diseases

基　　金：福建省科学技术厅重点项目(2009Y0018)

摘　　要：目的探讨内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)在蛛网膜下腔出血(sub-arachnoid hemorrhage,SAH)后不同时间点基底动脉表达情况及其与脑血管痉挛的关系。方法新西兰大白兔72只,随机分成两组:(1)对照组(12只);(2)SAH组(60只)。SAH组进一步按时间随机分为术后1d、3d、5d、7d、10d5个亚组,每组12只。采用枕大池二次注血法建立兔SAH模型。二次注血后在各个时间点采用灌流固定法处死动物,测定基底动脉横截面积判断有无脑血管痉挛。应用Western blotting分别观察eNOS在基底动脉中的表达情况。结果枕大池二次注血法成功制作SAH模型,基底动脉发生了不同程度的血管痉挛。Western blotting观察到eNOS在对照组大量表达,实验组第1天表达开始减少,第5天表达水平最低,之后表达逐渐增加。结论 SAH后eNOS表达水平的变化与脑血管痉挛发展的时相性具有一定的一致性,eNOS蛋白减少可能参与蛛网膜下腔出血后脑血管痉挛的发展。Objective To study the expression of endothelial nitric oxide synthase（eNOS） in the basilar artery after subarachnoid hemorrhage at various time points and the relationship between eNOS and cerebral vasospasm.Methods Seventy two New Zealand rabbits were randomized into 2 groups,control group（n=12） and SAH group（n=60）.Then the latter group was randomly divided into 5 subgroups according to the time points：1d,3d,5d,7d and 10d（12 per group）.Models of SAH were successfully induced via injecting autologous blood into the cisterna magna twice.The perfusion-fixation was performed for sacrifice of the rabbits in different time after the second blood injection in vivo.The lumen areas of the basilar artery were measured to assess vasospasm in all groups.The expression of eNOS protein in basilar artery was detected by Western-blotting.Results The SAH models that were established by injecting autologous blood into the cisterna magna twice were successful and basilar arteries demonstrated vasospasm to varying degrees.Western blotting indicated that the expression of eNOS protein in the control group was higher.In the SAH groups,it decreased on 1d,reached the lowest on 5d and increased in the later days.Conclusion There is a certain consistency between the expression changes of eNOS and CVS after SAH.The decrease of eNOS may play a role in the development of cerebral vasospasm after subarachnoid hemorrhage.

关 键 词：蛛网膜下腔出血 ENOS WESTERN BLOTTING 脑血管痉挛 

分 类 号：R743.35[医药卫生—神经病学与精神病学]