intermedin预处理对大鼠肾脏缺血再灌注损伤修复和再生过程的作用  

Effect of intermedin pretreatment on repair and regeneration process of renal ischemiareperfusion injury

作　　者：冯国徵[1] 李荣山[1] 乔晞[1] 孙秀丽[2] 

机构地区：[1]山西医科大学第二医院肾内科山西省肾脏病研究所,太原030001 [2]包头市中心医院肾内科

出　　处：《中华肾脏病杂志》2011年第12期917-922,共6页Chinese Journal of Nephrology

基　　金：国家自然科学基金（30971380）

摘　　要：目的探讨intermedin（IMD）预处理对大鼠肾脏缺血再灌注（IR）损伤修复和再生过程的作用。方法将Wistar大鼠按随机数字表法分为4组：假手术组（sham）、IR组、转空质粒组和转IMD组。在切除右肾后，转IMD组用超声微泡造影剂介导的基因转染方法将IMD真核质粒转染到大鼠肾组织，用RT-PCR和Western印迹法检测转染效率。转染成功后，制作。肾脏IR损伤模型，分别于再灌注后1d、2d、3d、4d、7d和14d6个时间点各取6只大鼠，留取血清及肾组织标本，常规检测血清BUN和Scr；HE和PAS染色观察肾组织的病理变化；免疫组化法观察肾小管上皮细胞的增殖程度。结果（1）转IMD组比转空质粒组的IMD蛋白和mRNA表达均增多（均P〈0．05），且转IMD组7d时表达最多，与转IMD组4d时差异无统计学意义；（2）与sham组相比，IR组1d和2d时Scr和BUN均显著增高（P〈0．05）；与IR组相比，转IMD组显著下降（P〈0．05）；转空质粒组与IR组相比差异无统计学意义（P〉0．05）。（3）IR组、转空质粒组和转IMD组大鼠的肾小管均受损，但转IMD组的损伤较轻，均以2d时病理损伤最重。（4）sham组肾小管和肾小球内几乎没有增殖细胞核抗原（PCNA）阳性细胞的表达；IR组和转空质粒组的PCNA阳性数在IR损伤1d时开始增加，7d时最多；转IMD组的PCNA阳性细胞数在IR损伤1d时开始增加，3d时最多。与IR组1-4d相比，转IMD组的PCNA阳性细胞数显著增加（P〈0．05）；与IR组7d相比，转IMD组7d的PCNA阳性细胞数显著减少（P〈0．05）。结论IMD预处理可以促进肾小管上皮细胞增殖，加速肾脏IR损伤修复和再生。Objective To explore the effect of intermedin pretreatment on repair and regeneration process of renal ischemia reperfusion （IR） injury. Methods Male Wistar rats were randomly divided into four groups： sham group, IR group, empty plasmid group and IMD group. One week after removing the right kidney, eukaryotic expression vector encoding rat IMD gene was transfected into the left kidney using an uhrasound-microbubble-mediated system, and transfection effect was detected by Western blotting and RT-PCR. After successful transfection, renal IR injury model was induced by clamping left renal arteries for 45 minutes followed by reperfusion. BUN and Scr were tested. Renal pathology was observed by PAS and HE staining. The proliferation oftubular epithelial cells was detected by SABC immunohistochemical method. Results （1）The expression of IMD protein and mRNA in IMD group was significantly up-regulated compared to empty plasmid group （P〈0.05）, reaching the peak at day 7, and the difference was not significant with that at day 4. （2）Compared with sham group, BUN and Scr were obviously increased at day 1 and 2 in IR group （P〈0.05）. However, compared with IR group, BUN and Scr in IMD group decreased obviously （P〈0.05）. Differences between IR group and empty plasmid group were not significant （P〉0.05）. （3）Kidney tubules in IR group, IMD group and empty plasmid group were injuryed, but the injury in IMD group was less serious compared with that in IR group and empty plasmid group. The injury in three groups was the most severe at day 2 after reperfusion. （4）The number of PCNA Positive cells evidently increased at day 7 in IR group and empty plasmid group, but the number of PCNA positive ceil evidently increased at day 3 in IMD group. Compared with IR group 1 to 4 days after perfusion, PCNA positive cells in IMD group increased obviously （P〈 0.05）. Compared with IR group day 7, PCNA expression in IMD group 7 d was obviously decreased （P〈 0.05）. Conclusion IMD pretreatm

关 键 词：再灌注损伤 转染 细胞增殖 修复和再生 INTERMEDIN 

分 类 号：R363[医药卫生—病理学]