Increased expression of granulocyte colony-stimulating factor mediates mesenchymal stem cells recruitment after vascular injury  

作　　者：ZHAO Yong LIU Ying-xi XIE Shuang-lun DENG Bing-qing WANG Jing-feng NIE Ru-qiong 

机构地区：[1]Department of Cardiology, Sun Yat-sen Memorial Hospital, SunYat-sen University, Guangzhou, Guangdong 510120, China

出　　处：《Chinese Medical Journal》2011年第24期4286-4292,共7页中华医学杂志（英文版）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. 30770899 to NIE Ru-qiong）, the Natural Science Foundation of Guangdong Province （No. 8151008901000072 to NIE Ru-qiong）.

摘　　要：Background Recent studies indicate that bone marrow-derived cells may significantly contribute to atherosclerosis, post-angioplasty restenosis and transplantation-associated vasculopathy. The responsible bone marrow （BM） cells and mechanisms regulating the mobilization of these cells are currently unclear. The purpose of this study was to investigate the expression of granulocyte colony-stimulating factor （G-CSF） on injured arteries and its effects on mesenchymal stem cells （MSCs） differentiation into vascular smooth muscle cells （VSMCs） in the process of vascular remodeling. Methods Balloon-mediated vascular injury was established in female rats （n=100） which received radioprotective whole female BM cells by tail vein injection and male MSCs through a tibial BM injection after lethal irradiation. The injured and contralateral carotid arteries were harvested at 3, 7, 14 and 28 days after treatment. Results Morphometric analysis indicated that intima to media area-ratio （I/M ratio） significantly increased at 28 days, 0.899＋0.057 （P 〈0.01）, compared with uninjured arteries. Combining fluorescence in situ hybridization （FISH） and immunohistochemical analysis showed that a significant number of the neointimal cells derived from MSCs, （45.2~8.5）% at 28 days （P=0.01）, compared with （23.5~6.3）% at 14 days. G-CSF was induced in carotid arteries subject to balloon angioplasty （fold mRNA change=8.67＋0.63 at three days, relative G-CSF protein=0.657±0.011 at three days, P 〈0.01, respectively, compared with uninjured arteries）. G-CSF was chemotactic for MSCs but did not affect the differentiation of MSCs into smooth-muscle-like cells. Conclusion Increased expression of G-CSF by injured arteries plays an essential role in contribution to recruitment and homing of MSCs to the site of the arterial lesion.Background Recent studies indicate that bone marrow-derived cells may significantly contribute to atherosclerosis, post-angioplasty restenosis and transplantation-associated vasculopathy. The responsible bone marrow （BM） cells and mechanisms regulating the mobilization of these cells are currently unclear. The purpose of this study was to investigate the expression of granulocyte colony-stimulating factor （G-CSF） on injured arteries and its effects on mesenchymal stem cells （MSCs） differentiation into vascular smooth muscle cells （VSMCs） in the process of vascular remodeling. Methods Balloon-mediated vascular injury was established in female rats （n=100） which received radioprotective whole female BM cells by tail vein injection and male MSCs through a tibial BM injection after lethal irradiation. The injured and contralateral carotid arteries were harvested at 3, 7, 14 and 28 days after treatment. Results Morphometric analysis indicated that intima to media area-ratio （I/M ratio） significantly increased at 28 days, 0.899＋0.057 （P 〈0.01）, compared with uninjured arteries. Combining fluorescence in situ hybridization （FISH） and immunohistochemical analysis showed that a significant number of the neointimal cells derived from MSCs, （45.2~8.5）% at 28 days （P=0.01）, compared with （23.5~6.3）% at 14 days. G-CSF was induced in carotid arteries subject to balloon angioplasty （fold mRNA change=8.67＋0.63 at three days, relative G-CSF protein=0.657±0.011 at three days, P 〈0.01, respectively, compared with uninjured arteries）. G-CSF was chemotactic for MSCs but did not affect the differentiation of MSCs into smooth-muscle-like cells. Conclusion Increased expression of G-CSF by injured arteries plays an essential role in contribution to recruitment and homing of MSCs to the site of the arterial lesion.

关 键 词：mesenchymal stem cells smooth muscle cells granulocyte colony-stimulating factor NEOINTIMA 

分 类 号：R543.5[医药卫生—心血管疾病]