紫外诱变选育高产蛋白酶枯草芽孢杆菌  

Selection of high protease-producing strain of Bacillus subtilis by UV mutation

作　　者：牛春华[1] 高岩[2] 李玉秋[1] 王成业[2] 王景会[1,2] 

机构地区：[1]吉林省农业科学院农产品加工研究中心,吉林长春130033 [2]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《中国酿造》2011年第12期67-69,共3页China Brewing

基　　金：吉林省科学技术厅资助项目(20090248)

摘　　要：紫外线辐射是目前最为广泛的物理诱变因子之一,其引起菌体DNA结构变化的形式很多,如DNA链的断裂、碱基的破坏等,但最主要的作用是使同链DNA的相邻嘧啶间形成胸腺嘧啶二聚体,阻碍碱基间的正常配对,从而引起微生物突变或死亡。以实验室保存的枯草芽孢杆菌为出发菌株,利用紫外诱变的方法,以获取可高效发酵生产蛋白酶的突变株。通过大量筛选及连续传代实验,确定了一株突变性状能稳定遗传的突变株,其蛋白酶产量较出发菌株有大幅度提高。UV radiation is one of common physical mutagenic method. It can make the same strand of DNA form thymine dimers between adjacent pyrimidine and hinder the base normally pair, and thus cause mutations or death of microorganisms. The Bacillus subtilis preserved by our laboratory was used as the original strain to be mutated by UV mutagenesis in order to increase the output of protease. One mutant strain was obtained, which was steady in hereditary. The yield ofprotease by the mutated strain was largely higher than that of parent strain.

关 键 词：紫外诱变 枯草芽孢杆菌:蛋白酶 

分 类 号：Q93-331[生物学—微生物学]