维生素B_6对实验性肝癌大鼠脂质过氧化的影响  

EFFECT OF VITAMIN B_6 ON LIPID PEROXIDATION IN 3′-MEDAB-INDUCED RATS

作　　者：赵会军[1] 张乃哲[1] 张敬一[1] 付琳杰[1] 赵军旭[2] 

机构地区：[1]河北省医学科学院,河北石家庄050021 [2]河北省药品检验所,河北石家庄050017

出　　处：《肿瘤研究与临床》2000年第1期11-13,共3页Cancer Research and Clinic

基　　金：河北省卫生厅资助项目!980 2 8

摘　　要：目的:探讨维生素B6对大鼠实验性肝癌形成过程中脂质过氧化的影响。方法:雄性SD大鼠随机分为三组。正常组大鼠喂普通饲料;DAB组大鼠饲料中含0.06%3′-甲基-4-双甲氨基偶氮苯(3′-MeDAB),10周后改喂普遍饲料;B6组大鼠饲料中除含有0.06%3′-MeDAB外,另加Vit.B6800mg/kg,10周后停喂3′-MeDAB,改喂加入800mg/kgVit.B6的普通饲料。动态观察大鼠全血GSH-Px的活性、血清MDA含量的改变。结果:(1)给予3′-MeDAB后,大鼠血中GSH-Px活性降低,MDA含量增加,与正常组相比有显著差异(P<0.01)。添加维生素B6后,在第45天,GSH-Px活性比DAB组明显提高(P<0.01),MDA含量明显降低(P<0.05)。(2)第45天时,DAB组的肝脏脏器系数已显著高于正常组(P>0.05)。结论:在实验性肝癌的早期,维生素B6有提高大鼠体内抗氧化酶活性、减少脂质过氧化物的作用,从而在一定程度上减轻致癌剂对大鼠肝脏的损害。Objective:The effect of Vitamin B 6 on lipid peroxidation induced by 3′ methyl 4 dimethylaminoazobenzene(3′ MeDAB)in rats was studied.Methods:51 male SD rats(86 g～122 g) were randomly devided into three groups : Group A received basal diet as normal control , Group B received 0.06% 3′ MeDAB in diet for 10 consecutive weeks,then received basal diet;Group C received 0.06% 3′ MeDAB along with Vitamin B 6(800mg/kg diet)daily in food for 10 weeks,then received basal diet along with the same dose of Vitamin B 6 for another 10 weeks. The rats were killed on the 45 day,90 day,140 day respectively in the experiment.The blood samples were collected from the abdominal aorta of the exprimental rats respectively for measurement of gluthione perosidase(GSH Px) and MDA content.Results:The MDA content of Group B was significantly higher than that of Group A (P<0.01),while GSH Px was dramatically lower than that of the latter.Supplementation of Vitamin B 6 inhibited the increase of MDA in serum at the early stage of carcinogenesis and restored the values of GSH Px in the blood.Conclusion:These results suggested that Vitamin B 6 is protective in limiting the action of GSH Px and depressing the lipid peroxidation during the initiation phase of hepatocarcinogenesis.

关 键 词：维生素B6 实验性治疗 肝癌 脂质过氧化 

分 类 号：R73-36[医药卫生—肿瘤]