外周血PMN激活吞噬化学发光与原发性肝癌患者免疫功能变化  

The PMN-CL and the change of immune function in patients with primary hepatic cancer

作　　者：曹锐[1] 马文书[1] 李天星[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤科,重庆400042

出　　处：《中国肿瘤临床与康复》2000年第1期22-23,共2页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　探讨外周血多形核白细胞化学发光 (Polymorphonuclear Chemilluminescence ,PMN CL)及细胞因子活性改变与原发性肝癌的发病机制关系。方法　采用液体闪烁技术 (LSC)体外激活PMN生成、释放过氧化阴离子 ,诱导吞噬作用产生化学发光 ,并应用ELISA及RIA检测相关指标IL 2、sIL 2R、IL 8、TNF、SOD ,并以健康献血员为对照。结果　体内激活明显 ,基础CL升高 (P <0 .0 5 ) ,最大峰值低下 ,峰时延迟 (P <0 .0 1)。相关指标IL 9、sIL 2R、TNF、SOD显著高于对照组 (P <0 .0 1) ,IL 2低于对照组 (P <0 .0 5 )。结论　原发性肝癌患者外周血PMN化学发光变化 ,客观反应了细胞免疫功能水平 ,以及超氧离子的生成、释放与损伤状态。对深入研究原发性肝癌的发病机理具有重要意义。? Objective To explore the relationship of PMN CL and the change of cytokines activity with pathogenesis of primary hepatic cancer.Methods The PMN CL and IL 2,sIL 2R,Il 8 TNF and SOD in sera of 38 patients with primary hepatic cancer were detected by LSC(Liquid scintillation counting)、ELISA and RIA.Results The level of basic luminescence increased(P<0.05).Iym induced phagoluminescence maximal peak value decreased.The peak time was delayed remarkably(P<0.01).At the same time,the sIL 2R、IL 8、TNF、SOD were significantly higher than those of normal control (P<0.01),IL 2 was lower than that of normal control (P<0.05).Conclusions The change of PMN CL reflected the immunologic function of the patients and the state of production,release of hyperoxide anion which might be an important role in the research of pathogenesis of primary hepatic cancer. 〔

关 键 词：原发性肝癌 多形核白细胞 化学发光 细胞因子 

分 类 号：R735.7[医药卫生—肿瘤]