p16真核表达载体的构建及其对肝癌细胞生长的抑制作用  

Construction of pcDNA_3/p16 piasmid and its inhibitory role in the proliferation of hepatoma cell line

作　　者：郑世曦[1] 李孝圭 周利军 朱兴族 

机构地区：[1]中国科学院上海药物研究所,上海市200031 [2]中国科学院上海生理研究所,上海市200031

出　　处：《世界华人消化杂志》2000年第1期49-51,共3页World Chinese Journal of Digestology

基　　金：香港王宽诚教育基金

摘　　要：目的构建pcDNA_3/p16真核表达质粒并了解其对肝癌细胞BEL-7404生长的抑制作用。方法将p16 cDNA亚克隆至pcDNA_3真核表达载体上,并经脂质体介导转染至BEL-7404细胞中。应用MTT法和流式细胞仪分析转染细胞生长情况和细胞周期。结果重组pcDNA_3/p16表达质粒构建成功,经pcDNA_3/p16转染的BEL-7404细胞生长速度受到明显抑制,且细胞多停滞于G_0/G_1期。结论重组pcDNA_3/p16质粒能在BEL-7404细胞内表达,且能抑制BEL-7404细胞的生长。AIM To construct pcDNA3 p16 eukaryotic expression plasmid and to investigate its inhibitory role in the proliferation of hepatoma cell line BEL-7404. METHODS p16 cDNA was subcloned into Liposome. MTT method and flow cytometry were used to investigate cell viability and cell cycle of the transfected BEL-7404 cells. RESULTS pcDNA3/p16 eukaryotic expression plasmid was obtained. The cell viability of the transfected BEL-7404 cells was decreased and the percentage of the cell in G0/G1 phase was increased.CONCLUSION pcDNA3/p16 plasmid could be expressed in BEL-7404 cells and it could inhibit the proliferation of the BEL-7404 cells.

关 键 词：肝肿瘤 P16基因 基因治疗 脂质体 转染 

分 类 号：R735.7[医药卫生—肿瘤]